Product Features
- No toxic reagents or ethanol precipitation needed
- Process completes in as little as 8 minutes
- High RNA integrity with no degradation
- OD260/OD280 ratio typically 2.1 to 2.2
- Suitable for most animal tissues and cell samples
Introduction
This product utilizes a unique DNA removal/RNA spin column technology and a novel solution system, eliminating the need for toxic reagents such as phenol/chloroform and β-mercaptoethanol. It is suitable for the efficient extraction of high-purity, high-quality total RNA from various fresh or frozen animal tissues or cells, with an optimal total process time of only 8 minutes. The extracted total RNA is of high purity, with minimal residual genomic DNA, and free from protein and other contaminants, making it suitable for various downstream applications such as RT-PCR, RT-qPCR, RNA-seq library preparation, and microarray analysis.
Kit Components
- RLT Plus Lysis Buffer
- Deproteinization Solution
- Wash Buffer
- RNase-free H2O
- Spin Column and Collection Tube
Assay Procedure Summary
- Prepare your sample (cells or tissue) and add lysis buffer.
- Mix thoroughly until no clumps remain.
- Transfer the sample to a spin column and centrifuge.
- Collect the filtrate and add ethanol; mix well.
- Load the mixture onto a new spin column and centrifuge.
- Wash the column with deproteinization solution, then centrifuge.
- Wash the column twice with wash buffer, centrifuging each time.
- Remove excess wash buffer by centrifuging the empty column.
- Elute RNA by adding RNase-free water to the column.
- Collect purified RNA for use or storage.
Storage/Stability
Store entire kit at room temperature. Stable for 12 months if stored properly.