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Epidermal Growth Factor, Human Recombinant
Catalog #: 228-10360
Product Description
Specifications
| Size | 500 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | Q6QBS2 |
| Protein Name / Synonyms | Urogastrone, URG, EGF. |
| Expressed Region | NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4. |
| Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is <, 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.The EGF is purified by proprietary chromatographic techniques.
Specifications
Protein Content
EGF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.