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Fibroblast Growth Factor-Basic Bovine Recombinant
Catalog #: 228-10451
Product Description
Specifications
| Size | 50 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Bovine (cow) |
| Accession Number | P03969 |
| Protein Name / Synonyms | HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser. |
| Expression System | E. coli |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% HSA. |
| Activity | The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <,0.1ng/ml, corresponding to a specific activity of 3 x 106 Units/mg. |
| Research Area | Angiogenesis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques.
Specifications
Protein Content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.