Exosome Services for Advanced Research

Exosomes are nanosized extracellular vesicles (30–150 nm) released by nearly all cell types and play a central role in intercellular communication. They transport functional biomolecules—including proteins, mRNA, miRNA, and lipids—to recipient cells, influencing pathways involved in tissue regeneration, immune modulation, inflammation, and disease progression. Because of their natural biocompatibility, stability, and targeting specificity, exosomes have emerged as powerful platforms for therapeutic development and drug delivery. In addition, exosomes reflect the molecular composition of their cells of origin and are readily detectable in biofluids, making them highly informative, minimally invasive biomarkers for disease diagnosis, prognosis, and treatment monitoring.

RayBiotech provides end-to-end services supporting both basic and translational research with reliable, scalable, and well-characterized solutions. Whether your work centers on mechanistic studies, diagnostic development, or therapeutic engineering, our solutions offer the reliability and flexibility needed to advance your research with confidence.

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Exosome Counting and Quality Assessment

Exosome Phenotyping

Exosome Production

Exosome Counting and Quality Assessment

We offer specialized services for accurate exosome quantification and quality assessment using flow cytometry–based methods. Our approach integrates purified exosome preparations, fluorescence labeling, and bead-based reference standards to deliver reliable, reproducible results for research and assay development.

Exosome Quantification by Flow Cytometry
Purified exosomes are fluorescently labeled with CFSE for sensitive detection by flow cytometry. Labeled samples are analyzed alongside reference beads of known concentration, enabling precise particle counting and normalization across samples. This bead-based approach improves accuracy compared to relative or signal-only measurements and supports cross-experiment comparability.
Quality Assessment and Data Confidence
Flow cytometry analysis provides insight into exosome integrity, labeling efficiency, and sample consistency. By combining CFSE labeling with internal bead standards, we help ensure that exosome measurements reflect true particle counts rather than instrument variability or signal drift.

Exosome Counting and Quality Assessment Image

Exosome identification by flow cytometry. A) Fluorescent reference beads sized from 0.02 µm to 2 µm are used to define the exosome size region based on side scatter and fluorescence signal. B) CFSE labeling enables clear discrimination of exosomes from background debris, revealing a distinct CFSE-positive population at <0.2 µm corresponding to exosomes.

Exosome Phenotyping

We provide flexible exosome phenotyping services, including traditional surface marker profiling and bead-based staining and analysis. These workflows are designed to support a broad range of applications, from basic characterization to functional and disease-focused studies.

Surface Marker Profiling
Exosome surface markers provide critical insight into origin, identity, and biological function. Our surface marker profiling services enable researchers to assess exosome quality, compare populations, and evaluate biologically relevant phenotypes. Researchers may select specific markers of interest or collaborate with our team to design a customized panel aligned with defined research goals.
Bead-Based Exosome Staining & Analysis
Bead-based staining enables high-confidence identification and comparative analysis of exosome surface markers by coupling vesicles to antibody-coated beads for flow cytometric detection.

Workflow:
1. PMMA beads conjugated with CD9, CD63, and CD81 antibodies*
2. Incubation with exosome samples
3. Fluorescent staining with antibodies against CD9, CD63, and CD81
4. Flow cytometric analysis with isotype controls

*CD9, CD63, and CD81 are commonly expressed exosome markers. Panels can be customized to target additional or alternative markers based on specific research needs.

Bead-based phenotyping of MSC-derived exosomes by flow cytometry. MSC-derived exosomes were captured on antibody-coated PMMA Beads and analyzed for canonical surface markers CD63, CD81, and CD9. Following incubation with exosome samples (PBS used as a negative control), captured vesicles were detected using a mixture of fluorophore-conjugated detection antibodies, with matched isotype controls to confirm specificity. By coupling exosomes to beads, this approach amplifies signal strength and enables clear, side-by-side comparison of marker expression, delivering confident and reproducible exosome phenotyping by flow cytometry.

Exosome Production

RayBiotech offers comprehensive exosome production services, including cell culture and expansion as well as exosome isolation and purification. Post-purification exosome counting and quality assessment can also be performed using the methods described above to ensure consistency and reproducibility.

Cell Culture Platforms
We employ optimized culture conditions using exosome-depleted media to ensure product purity and reproducibility.

Small-Scale Culture

Controlled cell expansion and conditioned media harvesting

Large-Scale Bioreactor Production

Stirred-Tank Bioreactors
- Ideal for suspension cell cultures
- Supports high cell density with real-time monitoring
Stirred-Tank Bioreactors with Microcarriers
- Optimized for adherent cell cultures
- High yield per volume
- Preferred for MSC- and stem cell-derived therapeutic exosomes

Isolation and Purification Platforms
RayBiotech utilizes hybrid platforms that integrate multiple isolation and purification strategies to maximize yield, purity, and scalability.

Polymer Precipitation–SEC Workflow:

PEG precipitation → SEC → sterile filtration

High yield (60–85%)
High purity
Scalable and continuous processing

Polymer Precipitation–Affinity Workflow:

PEG precipitation → antibody/ligand capture → elution → sterile filtration

Selective purification of specific exosome subpopulations
High specificity with moderate yield
Suitable for targeted and engineered exosomes

Discuss your project requirements

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Exosome Production

Cell Culture Platforms
We employ optimized culture conditions using exosome-depleted media to ensure product purity and reproducibility.

Small-Scale Culture

Controlled cell expansion and conditioned media harvesting

Large-Scale Bioreactor Production

Stirred-Tank Bioreactors
- Ideal for suspension cell cultures
- Supports high cell density with real-time monitoring
Stirred-Tank Bioreactors with Microcarriers
- Optimized for adherent cell cultures
- High yield per volume
- Preferred for MSC- and stem cell-derived therapeutic exosomes

Isolation and Purification Platforms
RayBiotech utilizes hybrid platforms that integrate multiple isolation and purification strategies to maximize yield, purity, and scalability.

Polymer Precipitation–SEC Workflow:

PEG precipitation → SEC → sterile filtration

High yield (60–85%)
High purity
Scalable and continuous processing

Polymer Precipitation–Affinity Workflow:

PEG precipitation → antibody/ligand capture → elution → sterile filtration

Selective purification of specific exosome subpopulations
High specificity with moderate yield
Suitable for targeted and engineered exosomes

Featured Products

Exosome Services for Advanced Research

Exosome Counting and Quality Assessment

Exosome Phenotyping

Exosome Production

Exosome Counting and Quality Assessment

Exosome Phenotyping

Exosome Production

Discuss your project requirements

Featured Products

Exosome Services for Advanced Research

Exosome Counting and Quality Assessment

Exosome Phenotyping

Exosome Production

Exosome Counting and Quality Assessment

Exosome Phenotyping

Exosome Production

Discuss your project requirements