RayBio® Human/Mouse/Rat Galanin-like peptide EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse, Rat |
| Sensitivity | 2.4 ng/ml |
| Detection Range | 0.1-1,000 ng/ml |
| Specificity | This EIA kit is designed to detect human, mouse, and rat Galanin-like peptide |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | Human: 2x / Mouse: 2x / Rat: 2x |
| Accession Number | Q9UBC7 |
| Gene Id | 85569 |
| Gene Symbols | GALP |
| Protein Name / Synonyms | Galanin-like peptide |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Competition-based |
| Method Of Detection | Colorimetric |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Galanin-like peptide (GALP) is a 60-amino acid neuropeptide was first isolated from the porcine hypothalamus. GALP shares sequence homology to galanin and can bind to and activate the three galanin receptor subtypes (GalR1-3). GALP is produced in the arcuate nucleus of the hypothalamus and the posterior pituitary gland, and can be detected in the blood. It is involved in the regulation of food intake, energy metabolism, reproduction and fluid intake. Recent reports have also suggested that GALP may play roles in epilepsy, Alzheimer's disease and diabetes, in addition to serving a vasoactive function.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.