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ISO 13485:2016 cGMP
Gliadin Gamma 18.6kD Wheat Recombinant
Catalog #: 228-12870
Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Plant |
| Expression System | E. coli |
| Purity | Greater than 94% as determined by SDS-PAGE. |
| Format | Sterile Filtered clear solution. |
| Formulation | Gliadin is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl, and 20% glycerol. |
| Recommended Applications | Western blot with monoclonal anti-hexa-His-tag antibody. < Standard ELISA test (checkerboard analysis of positive/negative sera panels), immunodot. (0.15-0.5 ug/ml depending on the type of ELISA plate and coating buffer). Binds IgG- and IgA-type h, Western blot with monoclonal anti-hexa-His-tag antibody. < Standard ELISA test (checkerboard analysis of positive/negative sera panels), immunodot. (0.15-0.5 ug/ml depending on the type of ELISA plate and coating buffer). Binds IgG- and IgA-type human autoantibodies associated with celiac disease. |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.