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Glucose-6-Phosphate Dehydrogenase, Human Recombinant
Catalog #: 228-20555
Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P11413 |
| Gene Id | 2539 |
| Protein Name / Synonyms | G6PD, G6PD1, Glucose-6-phosphate 1-dehydrogenase. |
| Expressed Region | MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE |
| Format | Sterile Filtered colorless solution. |
| Formulation | The G6PD solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl and 20% glycerol. |
| Activity | Specific activity is > 45 units/ml by measuring the increase of NADPH at 340 nm resulting from the reduction of β NADP. One unit oxidizes 1.0 µmole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of β-NADP at pH 7.4 at 25°C. |
| Research Area | Energy Metabolism, Glycolysis & Lipid Metabolism |
| Source | Hi-5 cells |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Description
G6PD Human Recombinant produced in Hi-5 cells is a single polypeptide chain containing 535 amino acids (1-515) and having a molecular mass of 61.4kDa.G6PD is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.