Chromatographically purified; assayed using NAD. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities ² 0.011%, 0.002%, 0.0011% and 0.0011% respectively. A lyophilized powder.

108.6 kDa (Theoretical)

≥360 NAD units per mg protein

Other Information

Extinction Coefficient

133,420 cm-1M-1 (Theoretical) E1%, 280.5 Equals 11.5, pH 7.2 in 0.1 M Tris-HCl (Olive and Levy 1971)

Isoelectric

4.6 (Olive and Levy 1971)

CATH

Two Domains: Class: Alpha Beta and Alpha Beta Architecture: 3-Layer(aba) Sandwich and 2-Layer Sandwich Topology: Rossmann fold and Dihydrodipicolinate Reductase (domain 2)

CAS Registry Number

C.A.S.: 9001-40-5

Storage Conditions

Store at 2-8°C.

108.6 kDa (Theoretical)

≥360 NAD units per mg protein

Other Information

Extinction Coefficient

133,420 cm-1M-1 (Theoretical) E1%, 280.5 Equals 11.5, pH 7.2 in 0.1 M Tris-HCl (Olive and Levy 1971)

Isoelectric

4.6 (Olive and Levy 1971)

CATH

Two Domains: Class: Alpha Beta and Alpha Beta Architecture: 3-Layer(aba) Sandwich and 2-Layer Sandwich Topology: Rossmann fold and Dihydrodipicolinate Reductase (domain 2)

CAS Registry Number

C.A.S.: 9001-40-5

Storage Conditions

Store at 2-8°C.

Size	2 kU, 18 KU
Estimated Lead Time	1-2 weeks
Accession Number	P11411
Expression System	Leuconostoc mesenteroides
Molecular Weight (kDa)	108.6 kDa (Theoretical)
Activity	≥360 NAD units per mg protein
Recommended Applications	Systems where NADPH or NADH is measured (Bhattacharya and Ali 1988) Determination of glucose and ATP (when coupled with HK), fructose, glucose-6-phosphate, NAD(P)+, and CK, Systems where NADPH or NADH is measured (Bhattacharya and Ali 1988) Determination of glucose and ATP (when coupled with HK), fructose, glucose-6-phosphate, NAD(P)+, and CK
Research Area	Phosphorylation, Energy Metabolism, Glycolysis & Lipid Metabolism
Source	Leuconostoc mesenteroides
Shipping Type	Blue ice
Storage	2-8°C

Size	2 kU, 18 KU
Estimated Lead Time	1-2 weeks
Accession Number	P11411
Expression System	Leuconostoc mesenteroides
Molecular Weight (kDa)	108.6 kDa (Theoretical)
Activity	≥360 NAD units per mg protein
Recommended Applications	Systems where NADPH or NADH is measured (Bhattacharya and Ali 1988) Determination of glucose and ATP (when coupled with HK), fructose, glucose-6-phosphate, NAD(P)+, and CK, Systems where NADPH or NADH is measured (Bhattacharya and Ali 1988) Determination of glucose and ATP (when coupled with HK), fructose, glucose-6-phosphate, NAD(P)+, and CK
Research Area	Phosphorylation, Energy Metabolism, Glycolysis & Lipid Metabolism
Source	Leuconostoc mesenteroides
Shipping Type	Blue ice
Storage	2-8°C

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