Target Protein
Principal Names
MRP5 antibody, DKFZp686C1782 antibody, ABCC5 antibody, ATP-binding cassette, sub-family C (CFTR/MRP), member 5 antibody, ABC33 antibody, EST277145 antibody, MOAT-C antibody, MOATC antibody, SMRP antibody, pABC11 antibody, ATP-binding cassette, sub-family C, member 5 antibody, canalicular multispecific organic anion transporter C antibody
Accession Number(s)
NP_005679.2; NP_001018881.1
Non-Human GeneID(s)
27416 (mouse), 116721 (rat)
Important Comments
This antibody is expected to recognize both reported isoforms (NP_005679.2; NP_001018881.1).
Immunogen
Peptide with sequence KDIDIGKEYIIP-C, from the N Terminus of the protein sequence according to NP_005679.2; NP_001018881.1.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Peptide ELISA
antibody detection limit dilution 1:32000.
Western blot
Approx. 150kDa band observed in Human Brain (Frnotal Cortex) and Mouse Brain lysates (calculated MW of 161kDa according to NP_005679.2). Recommended concentration: 0.5-1.5µg/ml.
IHC
In paraffin embedded Human Kidney shows membranous staining in DCT. Recommended concentration, 4-6µg/ml.
Species Reactivity
Expected from sequence similarity
Human, Mouse, Rat. Dog, Pig, Cow
Expiration:
12 months from the date of shipment when stored properly.