Recombinant Human GP73, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q8NBJ4 |
| Gene Id | 51280 |
| Gene Symbols | GOLM1 |
| Protein Name / Synonyms | Golgi Membrane Protein 1, Chromosome 9 Open Reading Frame 155, GOLPH2, HEL46, Golgi Phosphoprotein 2, PSEC0257, C9orf155, bA379P1.3, Golgi Membrane Protein GP73, Epididymis Luminal Protein 46, GP73, Golgi Protein 73-KD |
| Expressed Region | Ser36-Leu401 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human GP73 has a calculated molecular mass of 42 kDa. Due to the abundant glycosylation, it migrates as an approximately 60 kDa major protein band (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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