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ISO 13485:2016 cGMP
Granulocyte Macrophage-Colony Stimulating Factor, Human Recombinant
Catalog #: 228-10551
Product Description
Specifications
| Size | 20 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P04141 |
| Gene Id | 1437 |
| Protein Name / Synonyms | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | GM-CSF was lyophilized after extensive dialysis against 2mM sodium phosphate buffer pH= 7.4ñ0.1. |
| Activity | < 0.1 ng/ml |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques.
Specifications
Protein Content
GM-CSF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.