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Growth Hormone Pituitary 20kDa, Human Recombinant
Catalog #: 228-10538
Product Description
Specifications
| Size | 50 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P01241 |
| Gene Id | 2688 |
| Protein Name / Synonyms | GH1, GH, GHN, GH-N, hGH-N,Pituitary growth hormone, Growth hormone 1, Somatotropin. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | HGH-20K was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3 previously adjusted pH 10. |
| Research Area | Endocrine |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Growth Hormone 20KDa Pituitary Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20322 Dalton. HGH-20kDa is purified by proprietary chromatographic techniques.
Specifications
Protein Content
HGH-20K quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.735 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GH-20K as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Pituitary Growth Hormone-20K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH-20K can be stored at 4°C for up to 4 weeks. For long term storage and more diluted solutions it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.