PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF

Greater than 95% as determined by densitometric image analysis.

Sterile filtered colorless clear solution.

The HIV-1 Protease filtered (0.4um) solution (0.386mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.

Description

HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).

Kinetic parameters

Km=15.1 uM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) .. p-nitrophenylalanine).

Stability & Storage

Storage/Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF

Greater than 95% as determined by densitometric image analysis.

Sterile filtered colorless clear solution.

The HIV-1 Protease filtered (0.4um) solution (0.386mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.

Description

HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa).

Kinetic parameters

Km=15.1 uM, Kcat = 30s-1, Kcat/Km= 1981 mM-1s-1 with peptide substrate KARVF (NO2)VRKA (F(NO2) .. p-nitrophenylalanine).

Stability & Storage

Storage/Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles.

Size	10 µg, 1000 µg
Estimated Lead Time	2-3 weeks
Expressed Region	PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
Expression System	E. coli
Purity	Greater than 95% as determined by densitometric image analysis.
Format	Sterile filtered colorless clear solution.
Formulation	The HIV-1 Protease filtered (0.4um) solution (0.386mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.
Research Area	Infectious Disease, HIV
Shipping Type	Blue ice
Storage	-20°C

Size	10 µg, 1000 µg
Estimated Lead Time	2-3 weeks
Expressed Region	PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMNLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
Expression System	E. coli
Purity	Greater than 95% as determined by densitometric image analysis.
Format	Sterile filtered colorless clear solution.
Formulation	The HIV-1 Protease filtered (0.4um) solution (0.386mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.
Research Area	Infectious Disease, HIV
Shipping Type	Blue ice
Storage	-20°C

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