Taq DNA Polymerase is a heat-stable DNA polymerase derived from Thermus aquaticus YT-1, possessing both 5′→3′ polymerase activity and 5′→3′ exonuclease activity. The enzyme is modified with a specific antibody that inhibits its activity at low and room temperatures. Upon heating to 95°C, the antibody sheds, releasing the active enzyme. This hot-start mechanism prevents non-specific amplification caused by primer mismatches during reaction setup, thereby reducing the formation of non-specific products and primer dimers. As a result, the sensitivity and specificity of PCR amplification are significantly improved.

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into DNA in 30 minutes at 72℃ using M13 mp18 ssDNA as template/primer.

Includes template DNA, 0.5 μL each forward and reverse primer (10 μM), 0.5 μL dNTP Mix (10 mM each), 5 μL 5× HH Taq Buffer, 0.25 μL Hot Start Taq DNA Polymerase, and nuclease-free water up to 25 μL.

Initial denaturation: 95℃ for 1–3 min
Denaturation: 95℃ for 15–30 s (30–35 cycles)
Annealing: 55–65℃ for 15–60 s
Extension: 72℃, 1 kb/min
Final extension: 72℃ for 5 min

No detectable endonuclease, exonuclease, nickase, or RNase activity. E. coli DNA < 1 copy/5U confirmed by qPCR.

Ship with ice packs. Store at –20°C. Avoid repeated freeze-thaw cycles.