Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

TheHuman 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between 14-3-3 protein gamma and Cytokeratin-8 in the presence of potential inhibitors within 4 hours.

$931.00

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	6-8 weeks
Species	Human
Compatible Sample Types	Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number	P61981 / P05787
Gene Id	7532 / 3856
Gene Symbols	YWHAG / KRT8 CYK8
Protein Name / Synonyms	14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed] / Keratin, type II cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Research Area	Receptor-Ligand Binding
Shipping Type	Blue ice
Storage	4°C

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (BA-PRELIM.pdf)

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Featured Products

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Featured Products

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Human 14-3-3 protein gamma / Cytokeratin-8 Ligand Binding Assay Kit