Human Apoptosis Signaling Array C1

Membrane-based antibody array detects 19 phosphorylated or cleaved human apoptosis proteins. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	19
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Research Area	Apoptosis, HIF-1 alpha Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, mTOR Signaling, PI3K-AKT Signaling, TGF-beta Signaling, p53 Signaling, NFkB Signaling, Wnt-beta-Catenin Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472 Accession #: Q13315 ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	Caspase-3 (Cleaved Asp175) Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA	Caspase-7 (Cleaved Asp198) Gene Symbol: CASP7 MCH3 Gene ID: 840 Accession #: P55210
CHK1 (P-Ser296) Gene Symbol: CHEK1 CHK1 Gene ID: 1111 Accession #: O14757	CHK2 (P-Thr68) Gene Symbol: CHEK2 CDS1 CHK2 RAD53 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA	eIF-2a (P-Ser52) Gene Symbol: EIF2A CDA02 MSTP004 MSTP089 Gene ID: 83939 Accession #: Q9BY44 ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5595/5594 Accession #: P27361/P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792 Accession #: P25963 ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA	JNK (P-Thr183/Tyr185) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	NFKBP65 (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970 Accession #: Q04206 ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA	PARP1 (Cleaved Asp214/Gly215) Gene Symbol: PARP1 ADPRT PPOL Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
p38 (P-Thr180/Tyr182) Accession #: Q16539 ELISA Kit: Phospho-p38 alpha (T180/Y182) and Total p38 ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	SMAD2 (Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087 Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885 Accession #: O43318 ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with Staurosporine (STS) for 3 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

RayBiotech's Apoptosis Signaling Antibody Array identified increased NFkB activity in anoikis-resistant cancer cells. Figure adapted from Bao et al., Cancer Res, 2018.

RayBiotech's Apoptosis Signaling Antibody Array revealed ADS024’s anti-apoptotic effects in UCSE-treated colonic epithelial cells. Figure adapted from Irwin et al., Front Microbiol, 2024.

RayBiotech's Apoptosis Signaling Antibody Array showed isoginkgetin-induced apoptosis in U87MG glioblastoma cells. Figure adapted from Oliva et al., Molecules, 2022.

RayBiotech's Apoptosis Signaling Antibody Array demonstrated apoptosis induction in HepG2 cells treated with sorghum bran extract. Figure adapted from Smolensky et al., J Med Food, 2018.

Kit Components

Human Apoptosis Signaling Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (AAH-APOSIG-1.pdf)

SDS (8a2e613b43e9695c87002a0a1eefaf13c8aced0f.pdf)

Excel (e342344f86b64cd9f3ef98c999e29c820b6512dd.xls)

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	ATM (P-Ser1981) Gene Symbol: ATM Gene ID: 472 Accession #: Q13315 ELISA Kit: RayBio Human Phospho-ATM (S1981) ELISA	BAD (P-Ser112) Gene Symbol: BAD BBC6 BCL2L8 Gene ID: 572 Accession #: Q92934 ELISA Kit: RayBio Human Phospho-BAD (S112) ELISA	Caspase-3 (Cleaved Asp175) Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio Human/Mouse Cleaved-Caspase-3 (D175) ELISA	Caspase-7 (Cleaved Asp198) Gene Symbol: CASP7 MCH3 Gene ID: 840 Accession #: P55210
CHK1 (P-Ser296) Gene Symbol: CHEK1 CHK1 Gene ID: 1111 Accession #: O14757	CHK2 (P-Thr68) Gene Symbol: CHEK2 CDS1 CHK2 RAD53 Gene ID: 11200 Accession #: O96017 ELISA Kit: RayBio Human Phospho-Chk2 (T68) ELISA	eIF-2a (P-Ser52) Gene Symbol: EIF2A CDA02 MSTP004 MSTP089 Gene ID: 83939 Accession #: Q9BY44 ELISA Kit: RayBio Human Phospho-EIF2A (S52) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK3 ERK1 PRKM3/MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5595/5594 Accession #: P27361/P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phospho-HSP27 (S82) ELISA
IKBa (P-Ser32) Gene Symbol: NFKBIA IKBA MAD3 NFKBI Gene ID: 4792 Accession #: P25963 ELISA Kit: RayBio Human Phospho-I-kappa B alpha (S32) ELISA	JNK (P-Thr183/Tyr185) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	NFKBP65 (P-Ser536) Gene Symbol: RELA NFKB3 Gene ID: 5970 Accession #: Q04206 ELISA Kit: RayBio Human Phospho-NF-KB p65 (S536) ELISA	PARP1 (Cleaved Asp214/Gly215) Gene Symbol: PARP1 ADPRT PPOL Gene ID: 142 Accession #: P09874 ELISA Kit: RayBio Human Cleaved-PARP (D214/G215) ELISA	p27 (P-Thr198) Gene Symbol: PSMD9 Gene ID: 5715 Accession #: O00233 ELISA Kit: RayBio Human Phospho-P27/Kip1 (T198) ELISA
p38 (P-Thr180/Tyr182) Accession #: Q16539 ELISA Kit: Phospho-p38 alpha (T180/Y182) and Total p38 ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	SMAD2 (Ser245/250/255) Gene Symbol: SMAD2 MADH2 MADR2 Gene ID: 4087 Accession #: Q15796 ELISA Kit: RayBio Human/Mouse/Rat Phospho-SMAD2 (SER245/250/255) ELISA	TAK1 (P-Ser412) Gene Symbol: MAP3K7 TAK1 Gene ID: 6885 Accession #: O43318 ELISA Kit: RayBio Human Phospho-TAK1 (Ser412) ELISA

Typical Results

Jurkat cells were either untreated (bottom panel) or treated (top panel) with Camptothecin(CPT) for 16 hours. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

RayBiotech's Apoptosis Signaling Antibody Array identified increased NFkB activity in anoikis-resistant cancer cells. Figure adapted from Bao et al., Cancer Res, 2018.

RayBiotech's Apoptosis Signaling Antibody Array revealed ADS024’s anti-apoptotic effects in UCSE-treated colonic epithelial cells. Figure adapted from Irwin et al., Front Microbiol, 2024.

RayBiotech's Apoptosis Signaling Antibody Array showed isoginkgetin-induced apoptosis in U87MG glioblastoma cells. Figure adapted from Oliva et al., Molecules, 2022.

RayBiotech's Apoptosis Signaling Antibody Array demonstrated apoptosis induction in HepG2 cells treated with sorghum bran extract. Figure adapted from Smolensky et al., J Med Food, 2018.

Kit Components

Human Apoptosis Signaling Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

Great product
Rating
Was investigating ulcerative colitis serum exomes and drug ADS04 on HPECs and any downstream apoptosis. The kit was easy to use and gave a really good, clean output at the end
Review by Sophie, from UCLA, on Mar 13, 2023
Great product
Rating
The human apoptosis array provides a fast and comprehensive glance about the key signaling involved in the apoptotic pathway. It's a one shot experiment with certain controls already on the blot, pretty simple to execute and normalize. Would recommend it.
Review by Vinayak, from Moffitt Cancer Center, on Dec 4, 2019
Great product
Rating
This apoptosis kit was very user-friendly and gave detailed instructions on how to analyze findings using a software like Excel. This assay was very helpful for examining multiple gene targets simultaneously, so we got a lot of data out of 1 experiment. Negative and positive controls were included on the blots, which was useful in subsequent analyses.Notes: the Caspase3 antibody seemed a lot more sensitive than other antibodies on the blot. Also, some of the gene names in the kit manual differed from those listed on the website, so keep that in mind when analyzing data to ensure that the correct gene is being referred to.
Review by Melanie Gartz, from Medical College of Wisconsin, on Oct 8, 2019

Featured Products

Human Apoptosis Signaling Array C1

Product Description

Specifications

Product Features

Target Names

Typical Results

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Human Apoptosis Signaling Array C1

Product Description

Specifications

Product Features

Target Names

Typical Results

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

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Featured Products

Human Apoptosis Signaling Array C1

Product Description

Specifications

Product Features

Target Names

Typical Results

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human Apoptosis Signaling Array C1

Product Description

Specifications

Related Products

Product Features

Target Names

Typical Results

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Great product

Great product

Great product

Ask A Question

Great product

Great product

Great product

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