Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
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Scroll over each target protein for more information
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ATG12
- Gene Symbol: ATG12 APG12 APG12L
- Gene ID: 9140
- Accession #: O94817
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ATG7
- Gene Symbol: ATG7 APG7L
- Gene ID: 10533
- Accession #: O95352
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ATG10
- Gene Symbol: ATG10 APG10L PP12616
- Gene ID: 83734
- Accession #: Q9H0Y0
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ATG13
- Gene Symbol: ATG13 KIAA0652
- Gene ID: 9776
- Accession #: O75143
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ATG3
- Gene Symbol: ATG3 APG3 APG3L
- Gene ID: 64422
- Accession #: Q9NT62
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ATG4A
- Gene Symbol: ATG4A APG4A AUTL2
- Gene ID: 115201
- Accession #: Q8WYN0
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ATG4B
- Gene Symbol: ATG4B APG4B AUTL1 KIAA0943
- Gene ID: 23192
- Accession #: Q9Y4P1
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ATG5
- Gene Symbol: ATG5 APG5L ASP
- Gene ID: 9474
- Accession #: Q9H1Y0
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Beclin
- Gene Symbol: BECN1 GT197
- Gene ID: 8678
- Accession #: Q14457
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BNIP3L
- Gene Symbol: BNIP3L BNIP3A BNIP3H NIX
- Gene ID: 665
- Accession #: O60238
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DDR2
- Gene Symbol: DDR2 NTRKR3 TKT TYRO10
- Gene ID: 4921
- Accession #: Q16832
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GABARAP
- Gene Symbol: GABARAP FLC3B HT004
- Gene ID: 11337
- Accession #: O95166
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LC3a
- Gene Symbol: MAP1LC3A
- Gene ID: 84557
- Accession #: Q9H492
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LC3B
- Gene Symbol: MAP1LC3B MAP1ALC3
- Gene ID: 81631
- Accession #: Q9GZQ8
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NBS1
- Gene Symbol: NBN NBS NBS1 P95
- Gene ID: 4683
- Accession #: O60934
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P62
- Gene Symbol: SQSTM1 ORCA OSIL
- Gene ID: 8878
- Accession #: Q13501
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RHEB
- Gene Symbol: RHEB RHEB2
- Gene ID: 6009
- Accession #: Q15382
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MSK1
- Gene Symbol: RPS6KA5 MSK1
- Gene ID: 9252
- Accession #: O75582
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Alpha-synuclein
- Gene Symbol: SNCA NACP PARK1
- Gene ID: 6622
- Accession #: P37840
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Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Autophagy Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.