Human Cytokine Array C9

RayBio^® C-Series Human Cytokine Antibody Array 9 Kit. Detects 51 Human Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	51
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C
Storage / Stability	Easy to use No specialized equipment needed Compatible with nearly any liquid sample Proven technology (many publications) Highly sensitive (pg/ml) Sandwich ELISA specificity Higher density than ELISA, Western blot or bead-based multiplex
More Information	Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA	BCAM Gene Symbol: BCAM Gene ID: 4059 Accession #: P50895 ELISA Kit: RayBio Human BCAM ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	Fc gamma RIIB/C (CD32B/C) Gene Symbol: FCGR2B Gene ID: 2213 Accession #: P31994 ELISA Kit: RayBio Human FcgRIIBC ELISA
Ferritin Gene Symbol: FTH1 Gene ID: 2495 Accession #: P02794 ELISA Kit: RayBio Human Ferritin ELISA	FLRG Gene Symbol: FSTL3 Gene ID: 10272 Accession #: O95633 ELISA Kit: RayBio Human FLRG ELISA	Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	Furin Gene Symbol: Furin Gene ID: 5045 Accession #: P09958 ELISA Kit: RayBio Human Furin ELISA	Galectin-7 Gene Symbol: LGALS7 Gene ID: 3963 Accession #: P47929 ELISA Kit: RayBio Human Galectin-7 ELISA
GDF-15 Gene Symbol: GDF15 Gene ID: 9518 Accession #: Q99988 ELISA Kit: RayBio Human GDF-15 ELISA	Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	IL-10 R alpha Gene Symbol: IL10RA Gene ID: 3587 Accession #: Q13651 ELISA Kit: RayBio Human IL-10R Alpha ELISA	IL-22 Gene Symbol: IL22 Gene ID: 50616 Accession #: Q9GZX6 ELISA Kit: RayBio Human IL-22 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA
IL-29 (IFN-lambda 1) Gene Symbol: IFNL1 Gene ID: 282618 Accession #: Q8IU54 ELISA Kit: RayBio Human IL-29 ELISA	IL-31 Gene Symbol: IL31 Gene ID: 386653 Accession #: Q6EBC2 ELISA Kit: RayBio Human IL-31 ELISA	Insulin Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human Insulin ELISA	Luteinizing hormone Gene Symbol: LHB Gene ID: 3972 Accession #: P01229 ELISA Kit: RayBio Human LH ELISA	LIMPII Gene Symbol: SCARB2 Gene ID: 950 Accession #: Q14108 ELISA Kit: RayBio Human LIMP-II ELISA
LYVE-1 Gene Symbol: LYVE1 Gene ID: 10894 Accession #: Q9Y5Y7 ELISA Kit: RayBio Human LYVE-1 ELISA	Marapsin Gene Symbol: PRSS27 Gene ID: 83886 Accession #: Q9BQR3 ELISA Kit: RayBio Human Marapsin ELISA	MICA Gene Symbol: MICA Gene ID: 100507436 Accession #: Q29983 ELISA Kit: RayBio Human MICA ELISA	MICB Gene Symbol: MICB Gene ID: 4277 Accession #: Q29980 ELISA Kit: RayBio Human MICB ELISA	MMP-2 Gene Symbol: MMP2 Gene ID: 4313 Accession #: P08253 ELISA Kit: RayBio Human MMP-2 ELISA
MMP-7 Gene Symbol: MMP7 Gene ID: 4316 Accession #: P09237 ELISA Kit: RayBio Human MMP-7 ELISA	MMP-8 Gene Symbol: MMP8 Gene ID: 4317 Accession #: P22894 ELISA Kit: RayBio Human MMP-8 ELISA	MMP-10 Gene Symbol: MMP10 Gene ID: 4319 Accession #: P09238 ELISA Kit: RayBio Human MMP-10 ELISA	NCAM-1 (CD56) Gene Symbol: NCAM1 Gene ID: 4684 Accession #: P13591 ELISA Kit: RayBio Human NCAM-1 ELISA	Nidogen-1 Gene Symbol: NID1 Gene ID: 4811 Accession #: P14543 ELISA Kit: RayBio Human Nidogen-1 ELISA
NrCAM Gene Symbol: NrCAM Gene ID: 4897 Accession #: Q92823 ELISA Kit: RayBio Human NRCAM ELISA	NRG1-beta 1 (HRG1-beta 1) Gene Symbol: NRG1 Gene ID: 3084 Accession #: Q02297 ELISA Kit: RayBio Human NRG1-beta 1 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	PAI-1 Gene Symbol: SERPINE1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA	Platelet factor 4 (CXCL4) Gene Symbol: PF4 CXCL4 SCYB4 Gene ID: 5196 Accession #: P02776 ELISA Kit: RayBio Human PF-4 ELISA
PSA-total Gene Symbol: KLK3 Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA-total ELISA	RAGE Gene Symbol: MOK Gene ID: 5891 Accession #: Q9UQ07 ELISA Kit: RayBio Human RAGE ELISA	RANK (TNFRSF11A) Gene Symbol: TNFRSF11A Gene ID: 8792 Accession #: Q9Y6Q6 ELISA Kit: RayBio Human RANK ELISA	Resistin Gene Symbol: RETN Gene ID: 56729 Accession #: Q9HD89 ELISA Kit: RayBio Human Resistin ELISA	SAA (Serum Amyloid A) Gene Symbol: SAA1 Gene ID: 6288 Accession #: P0DJI8 ELISA Kit: RayBio Human SAA ELISA
Siglec-9 Gene Symbol: SIGLEC9 Gene ID: 27180 Accession #: Q9Y336 ELISA Kit: RayBio Human Siglec-9 ELISA	TACE Gene Symbol: TACE ELISA Kit: RayBio Human TACE ELISA	TIM-1 (KIM-1) Gene Symbol: HAVCR1 Gene ID: 26762 Accession #: Q96D42 ELISA Kit: RayBio Human TIM-1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA	Trappin-2 Gene Symbol: PI3 Gene ID: 5266 Accession #: P19957 ELISA Kit: RayBio Human Trappin-2 ELISA
TREM-1 Gene Symbol: TREM1 Gene ID: 54210 Accession #: Q9NP99 ELISA Kit: RayBio Human TREM-1 ELISA	TSH Gene Symbol: CGA; TSHB ELISA Kit: RayBio Human TSH ELISA	TSLP Gene Symbol: TSLP Gene ID: 85480 Accession #: Q969D9 ELISA Kit: RayBio Human TSLP ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA	VEGF-C Gene Symbol: VEGFC Gene ID: 7424 Accession #: P49767 ELISA Kit: RayBio Human VEGF-C ELISA
XEDAR Gene Symbol: EDA2R Gene ID: 60401 Accession #: Q9HAV5

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C9 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (AAH-CYT-9.pdf)

SDS (ad5295fbdd349d3df497c6bacb690af38650d431.pdf)

Excel (43762a98f64a4d3dc41cd4a81da1be4c6a298e95.xls)

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA	BCAM Gene Symbol: BCAM Gene ID: 4059 Accession #: P50895 ELISA Kit: RayBio Human BCAM ELISA	CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	Fc gamma RIIB/C (CD32B/C) Gene Symbol: FCGR2B Gene ID: 2213 Accession #: P31994 ELISA Kit: RayBio Human FcgRIIBC ELISA
Ferritin Gene Symbol: FTH1 Gene ID: 2495 Accession #: P02794 ELISA Kit: RayBio Human Ferritin ELISA	FLRG Gene Symbol: FSTL3 Gene ID: 10272 Accession #: O95633 ELISA Kit: RayBio Human FLRG ELISA	Follistatin Gene Symbol: FST Gene ID: 10468 Accession #: P19883 ELISA Kit: RayBio Human Follistatin ELISA	Furin Gene Symbol: Furin Gene ID: 5045 Accession #: P09958 ELISA Kit: RayBio Human Furin ELISA	Galectin-7 Gene Symbol: LGALS7 Gene ID: 3963 Accession #: P47929 ELISA Kit: RayBio Human Galectin-7 ELISA
GDF-15 Gene Symbol: GDF15 Gene ID: 9518 Accession #: Q99988 ELISA Kit: RayBio Human GDF-15 ELISA	Growth Hormone Gene Symbol: GH1 Gene ID: 2688 Accession #: P01241 ELISA Kit: RayBio Human GH ELISA	IL-10 R alpha Gene Symbol: IL10RA Gene ID: 3587 Accession #: Q13651 ELISA Kit: RayBio Human IL-10R Alpha ELISA	IL-22 Gene Symbol: IL22 Gene ID: 50616 Accession #: Q9GZX6 ELISA Kit: RayBio Human IL-22 ELISA	IL-28A (IFN-lambda 2) Gene Symbol: IFNL2 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA
IL-29 (IFN-lambda 1) Gene Symbol: IFNL1 Gene ID: 282618 Accession #: Q8IU54 ELISA Kit: RayBio Human IL-29 ELISA	IL-31 Gene Symbol: IL31 Gene ID: 386653 Accession #: Q6EBC2 ELISA Kit: RayBio Human IL-31 ELISA	Insulin Gene Symbol: INS Gene ID: 3630 Accession #: P01308 ELISA Kit: RayBio Human Insulin ELISA	Luteinizing hormone Gene Symbol: LHB Gene ID: 3972 Accession #: P01229 ELISA Kit: RayBio Human LH ELISA	LIMPII Gene Symbol: SCARB2 Gene ID: 950 Accession #: Q14108 ELISA Kit: RayBio Human LIMP-II ELISA
LYVE-1 Gene Symbol: LYVE1 Gene ID: 10894 Accession #: Q9Y5Y7 ELISA Kit: RayBio Human LYVE-1 ELISA	Marapsin Gene Symbol: PRSS27 Gene ID: 83886 Accession #: Q9BQR3 ELISA Kit: RayBio Human Marapsin ELISA	MICA Gene Symbol: MICA Gene ID: 100507436 Accession #: Q29983 ELISA Kit: RayBio Human MICA ELISA	MICB Gene Symbol: MICB Gene ID: 4277 Accession #: Q29980 ELISA Kit: RayBio Human MICB ELISA	MMP-2 Gene Symbol: MMP2 Gene ID: 4313 Accession #: P08253 ELISA Kit: RayBio Human MMP-2 ELISA
MMP-7 Gene Symbol: MMP7 Gene ID: 4316 Accession #: P09237 ELISA Kit: RayBio Human MMP-7 ELISA	MMP-8 Gene Symbol: MMP8 Gene ID: 4317 Accession #: P22894 ELISA Kit: RayBio Human MMP-8 ELISA	MMP-10 Gene Symbol: MMP10 Gene ID: 4319 Accession #: P09238 ELISA Kit: RayBio Human MMP-10 ELISA	NCAM-1 (CD56) Gene Symbol: NCAM1 Gene ID: 4684 Accession #: P13591 ELISA Kit: RayBio Human NCAM-1 ELISA	Nidogen-1 Gene Symbol: NID1 Gene ID: 4811 Accession #: P14543 ELISA Kit: RayBio Human Nidogen-1 ELISA
NrCAM Gene Symbol: NrCAM Gene ID: 4897 Accession #: Q92823 ELISA Kit: RayBio Human NRCAM ELISA	NRG1-beta 1 (HRG1-beta 1) Gene Symbol: NRG1 Gene ID: 3084 Accession #: Q02297 ELISA Kit: RayBio Human NRG1-beta 1 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	PAI-1 Gene Symbol: SERPINE1 Gene ID: 5054 Accession #: P05121 ELISA Kit: RayBio Human PAI-1 ELISA	Platelet factor 4 (CXCL4) Gene Symbol: PF4 CXCL4 SCYB4 Gene ID: 5196 Accession #: P02776 ELISA Kit: RayBio Human PF-4 ELISA
PSA-total Gene Symbol: KLK3 Gene ID: 354 Accession #: P07288 ELISA Kit: RayBio Human PSA-total ELISA	RAGE Gene Symbol: MOK Gene ID: 5891 Accession #: Q9UQ07 ELISA Kit: RayBio Human RAGE ELISA	RANK (TNFRSF11A) Gene Symbol: TNFRSF11A Gene ID: 8792 Accession #: Q9Y6Q6 ELISA Kit: RayBio Human RANK ELISA	Resistin Gene Symbol: RETN Gene ID: 56729 Accession #: Q9HD89 ELISA Kit: RayBio Human Resistin ELISA	SAA (Serum Amyloid A) Gene Symbol: SAA1 Gene ID: 6288 Accession #: P0DJI8 ELISA Kit: RayBio Human SAA ELISA
Siglec-9 Gene Symbol: SIGLEC9 Gene ID: 27180 Accession #: Q9Y336 ELISA Kit: RayBio Human Siglec-9 ELISA	TACE Gene Symbol: TACE ELISA Kit: RayBio Human TACE ELISA	TIM-1 (KIM-1) Gene Symbol: HAVCR1 Gene ID: 26762 Accession #: Q96D42 ELISA Kit: RayBio Human TIM-1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA	Trappin-2 Gene Symbol: PI3 Gene ID: 5266 Accession #: P19957 ELISA Kit: RayBio Human Trappin-2 ELISA
TREM-1 Gene Symbol: TREM1 Gene ID: 54210 Accession #: Q9NP99 ELISA Kit: RayBio Human TREM-1 ELISA	TSH Gene Symbol: CGA; TSHB ELISA Kit: RayBio Human TSH ELISA	TSLP Gene Symbol: TSLP Gene ID: 85480 Accession #: Q969D9 ELISA Kit: RayBio Human TSLP ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA	VEGF-C Gene Symbol: VEGFC Gene ID: 7424 Accession #: P49767 ELISA Kit: RayBio Human VEGF-C ELISA
XEDAR Gene Symbol: EDA2R Gene ID: 60401 Accession #: Q9HAV5

Species Detected

Human

Application Notes

Suggested Application

Kit Components

Human Cytokine Antibody Array C9 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Featured Products

Human Cytokine Array C9

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Human Cytokine Array C9

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Human Cytokine Array C9

Human Cytokine Array C9

Featured Products

Human Cytokine Array C9

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human Cytokine Array C9

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Human Cytokine Array C9

Human Cytokine Array C9