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Human Cytokine Array G4000

RayBio^® G-Series Human Cytokine Antibody Array 4000 Kit. A combination of Human Cytokine Antibody Array G6, G7, G8, G9, & G10. Detects 274 Human Cytokines. Suitable for all liquid sample types.

Product Description

Specifications

Size	4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	274
Solid Support	Glass Slide
Design Principle	Sandwich-based
Method Of Detection	Fluorescence Laser Scanner
Shipping Type	Blue ice
Storage	-20°C
Storage / Stability	High sensitivity and specificity Low sample volume (10-100 µl per array) Large dynamic range of detection Compatible with most sample types Test 4 or 8 samples on each slide Suitable for high-throughput assays
More Information	Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	ACE-2 Gene Symbol: ACE2 Gene ID: 59272 Accession #: Q9BYF1	Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA
AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	ALCAM (CD166) Gene Symbol: ALCAM Gene ID: 214 Accession #: Q13740 ELISA Kit: RayBio Human ALCAM ELISA	Alpha-fetoprotein Gene Symbol: AFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA
Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA
CD80 (B7-1) Gene Symbol: CD80 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80 ELISA	Beta-2 Microglobulin Gene Symbol: B2M Gene ID: 567 Accession #: P61769 ELISA Kit: RayBio Human B2M ELISA	BCAM Gene Symbol: BCAM Gene ID: 4059 Accession #: P50895 ELISA Kit: RayBio Human BCAM ELISA	BCMA (TNFRSF17) Gene Symbol: TNFRSF17 Gene ID: 608 Accession #: Q02223 ELISA Kit: RayBio Human TNFRSF17 ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA
beta IG-H3 Gene Symbol: TGFBI Gene ID: 7045 Accession #: Q15582 ELISA Kit: RayBio Human BIGH3 ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA
BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA	BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CA125 Gene Symbol: MUC16 Gene ID: 94025 Accession #: Q8WXI7 ELISA Kit: RayBio Human CA-125 ELISA
CA15-3 Gene Symbol: MUC1 Gene ID: 4582 Accession #: P15941 ELISA Kit: RayBio Human CA15-3 ELISA	CA19-9 ELISA Kit: RayBio Human CA19-9 ELISA	CA9 Gene Symbol: CA9 Gene ID: 768 Accession #: Q16790 ELISA Kit: RayBio Human CA9 ELISA	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: 1489 Accession #: Q16619 ELISA Kit: RayBio Human CT-1 ELISA	Cathepsin S Gene Symbol: CTSS Gene ID: 1520 Accession #: P25774 ELISA Kit: RayBio Human Cathepsin S ELISA
HCC-1 (CCL14) Gene Symbol: CCL14 Gene ID: 6358 Accession #: Q16627 ELISA Kit: RayBio Human HCC-1 ELISA	6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: 6366 Accession #: O00585 ELISA Kit: RayBio Human 6Ckine ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CD14 Gene Symbol: CD14 Gene ID: 929 Accession #: P08571 ELISA Kit: RayBio Human CD14 ELISA	CD23 Gene Symbol: FCER2 Gene ID: 2208 Accession #: P06734 ELISA Kit: RayBio Human CD23 ELISA
CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CEA ELISA Kit: RayBio Human CEA ELISA	CEACAM-1 Gene Symbol: CEACAM1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA
CK beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	CNTF Gene Symbol: CNTF Gene ID: 1270 Accession #: P26441 ELISA Kit: RayBio Human CNTF ELISA	Cripto-1 Gene Symbol: TDGF1 Gene ID: 6997 Accession #: P13385	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA
CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	DAN Gene Symbol: PARN Gene ID: 5073 Accession #: O95453 ELISA Kit: RayBio Human DAN ELISA	Decorin Gene Symbol: DCN Gene ID: 1634 Accession #: P07585 ELISA Kit: RayBio Human Decorin ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA	Dkk-3 Gene Symbol: DKK3 Gene ID: 27122 Accession #: Q9UBP4 ELISA Kit: RayBio Human Dkk-3 ELISA
Dkk-4 Gene Symbol: DKK4 Gene ID: 27121 Accession #: Q9UBT3 ELISA Kit: RayBio Human Dkk-4 ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: 1803 Accession #: P27487 ELISA Kit: RayBio Human CD26 ELISA	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA
EDA-A2 Gene Symbol: EDA Gene ID: 1896 Accession #: Q92838 ELISA Kit: RayBio Human EDA-A2 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA	EG-VEGF (PK1) Gene Symbol: PROK1 Gene ID: 84432 Accession #: P58294 ELISA Kit: RayBio Human EG-VEGF ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA
Endoglin (CD105) Gene Symbol: ENG Gene ID: 2022 Accession #: P17813 ELISA Kit: RayBio Human Endoglin ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array G4000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (AAH-CYT-G4000.pdf)

SDS (983ed7dfb17cf6b115a3a4d36521cc569490ceed.pdf)

GAL (74447edd71e5607d36c10911696d17a00890bf7f.gal)

GAL (47344738ae29eab4cf3efdb288dea102d6c0f195.gal)

GAL (49378e3ffa5c4ca437d6e9a89e4ff6c0f397fbab.gal)

GAL (2cf82ec32823cf974d27c5ce9d76bc6d0c1dc175.gal)

GAL (58321ff6c6896b5c5973e00e3884aeb028842a83.gal)

GAL (db0fbb3d078118d44ac59964f84ca5dbc66f44dc.gal)

GAL (b6cc7248dc50abf91363f9d332a6206755213b81.gal)

GAL (96d2f7934f84463176badd9576263b438f0cb14f.gal)

GAL (8740bf3764568718c9451bd3d50720dd599767d4.gal)

GAL (d2d1c8569b8fd0b31d4c7e042ce959e4fce28d08.gal)

Excel (e6df819a7ca617044551182c6298f8c2e34792ab.xls)

Excel (419d62e88e68d42f3c3fa61a67e10189fd2aa209.xls)

Excel (c34f9e6cecc3e21354a83d2982f81f26be48d699.xls)

Excel (9e542377999120f0382d72d0d04495dd3a8bbb05.xls)

Excel (28e9b9ca53735943b724cd055c1240ba9ddbfe39.xls)

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
4-1BB (TNFRSF9/CD137) Gene Symbol: TNFRSF9 Gene ID: 3604 Accession #: Q07011 ELISA Kit: RayBio Human TNFRSF9 ELISA	ACE-2 Gene Symbol: ACE2 Gene ID: 59272 Accession #: Q9BYF1	Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	Adipsin (Complement Factor D) Gene Symbol: CFD Gene ID: 1675 Accession #: P00746 ELISA Kit: RayBio Human Adipsin ELISA
AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	ALCAM (CD166) Gene Symbol: ALCAM Gene ID: 214 Accession #: Q13740 ELISA Kit: RayBio Human ALCAM ELISA	Alpha-fetoprotein Gene Symbol: AFP Gene ID: 174 Accession #: P02771 ELISA Kit: RayBio Human AFP ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA
Angiopoietin-1 Gene Symbol: ANGPT1 Gene ID: 284 Accession #: Q15389 ELISA Kit: RayBio Human ANGPT1 ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA	ANGPTL4 Gene Symbol: ANGPTL4 Gene ID: 51129 Accession #: Q9BY76 ELISA Kit: RayBio Human ANGPTL4 ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA
CD80 (B7-1) Gene Symbol: CD80 Gene ID: 941 Accession #: P33681 ELISA Kit: RayBio Human CD80 ELISA	Beta-2 Microglobulin Gene Symbol: B2M Gene ID: 567 Accession #: P61769 ELISA Kit: RayBio Human B2M ELISA	BCAM Gene Symbol: BCAM Gene ID: 4059 Accession #: P50895 ELISA Kit: RayBio Human BCAM ELISA	BCMA (TNFRSF17) Gene Symbol: TNFRSF17 Gene ID: 608 Accession #: Q02223 ELISA Kit: RayBio Human TNFRSF17 ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA
beta IG-H3 Gene Symbol: TGFBI Gene ID: 7045 Accession #: Q15582 ELISA Kit: RayBio Human BIGH3 ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA	BMP-5 Gene Symbol: BMP5 Gene ID: 653 Accession #: P22003 ELISA Kit: RayBio Human BMP-5 ELISA
BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA	BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CA125 Gene Symbol: MUC16 Gene ID: 94025 Accession #: Q8WXI7 ELISA Kit: RayBio Human CA-125 ELISA
CA15-3 Gene Symbol: MUC1 Gene ID: 4582 Accession #: P15941 ELISA Kit: RayBio Human CA15-3 ELISA	CA19-9 ELISA Kit: RayBio Human CA19-9 ELISA	CA9 Gene Symbol: CA9 Gene ID: 768 Accession #: Q16790 ELISA Kit: RayBio Human CA9 ELISA	Cardiotrophin-1 (CT-1) Gene Symbol: CTF1 Gene ID: 1489 Accession #: Q16619 ELISA Kit: RayBio Human CT-1 ELISA	Cathepsin S Gene Symbol: CTSS Gene ID: 1520 Accession #: P25774 ELISA Kit: RayBio Human Cathepsin S ELISA
HCC-1 (CCL14) Gene Symbol: CCL14 Gene ID: 6358 Accession #: Q16627 ELISA Kit: RayBio Human HCC-1 ELISA	6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: 6366 Accession #: O00585 ELISA Kit: RayBio Human 6Ckine ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CD14 Gene Symbol: CD14 Gene ID: 929 Accession #: P08571 ELISA Kit: RayBio Human CD14 ELISA	CD23 Gene Symbol: FCER2 Gene ID: 2208 Accession #: P06734 ELISA Kit: RayBio Human CD23 ELISA
CD30 (TNFRSF8) Gene Symbol: CD30 Gene ID: 943 Accession #: P28908 ELISA Kit: RayBio Human CD30 ELISA	CD40 (TNFRSF5) Gene Symbol: CD40 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA	CEA ELISA Kit: RayBio Human CEA ELISA	CEACAM-1 Gene Symbol: CEACAM1 Gene ID: 634 Accession #: P13688 ELISA Kit: RayBio Human CEACAM-1 ELISA
CK beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA	CNTF Gene Symbol: CNTF Gene ID: 1270 Accession #: P26441 ELISA Kit: RayBio Human CNTF ELISA	Cripto-1 Gene Symbol: TDGF1 Gene ID: 6997 Accession #: P13385	CRP (C-Reactive Protein) Gene Symbol: CRP Gene ID: 1401 Accession #: P02741 ELISA Kit: RayBio Human CRP ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA
CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	DAN Gene Symbol: PARN Gene ID: 5073 Accession #: O95453 ELISA Kit: RayBio Human DAN ELISA	Decorin Gene Symbol: DCN Gene ID: 1634 Accession #: P07585 ELISA Kit: RayBio Human Decorin ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA	Dkk-3 Gene Symbol: DKK3 Gene ID: 27122 Accession #: Q9UBP4 ELISA Kit: RayBio Human Dkk-3 ELISA
Dkk-4 Gene Symbol: DKK4 Gene ID: 27121 Accession #: Q9UBT3 ELISA Kit: RayBio Human Dkk-4 ELISA	CD26 (DPPIV) Gene Symbol: DPP4 Gene ID: 1803 Accession #: P27487 ELISA Kit: RayBio Human CD26 ELISA	DR6 (TNFRSF21) Gene Symbol: TNFRSF21 Gene ID: 27242 Accession #: O75509 ELISA Kit: RayBio Human DR6 ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	E-Cadherin Gene Symbol: CDH1 Gene ID: 999 Accession #: P12830 ELISA Kit: RayBio Human E-Cadherin ELISA
EDA-A2 Gene Symbol: EDA Gene ID: 1896 Accession #: Q92838 ELISA Kit: RayBio Human EDA-A2 ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA	EG-VEGF (PK1) Gene Symbol: PROK1 Gene ID: 84432 Accession #: P58294 ELISA Kit: RayBio Human EG-VEGF ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA
Endoglin (CD105) Gene Symbol: ENG Gene ID: 2022 Accession #: P17813 ELISA Kit: RayBio Human Endoglin ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	TROP1 (EpCAM) Gene Symbol: EPCAM Gene ID: 4072 Accession #: P16422 ELISA Kit: RayBio Human TROP1 ELISA

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array G4000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Featured Products

Human Cytokine Array G4000

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Human Cytokine Array G4000

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Have this product in hand?

Human Cytokine Array G4000

Human Cytokine Array G4000

Featured Products

Human Cytokine Array G4000

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human Cytokine Array G4000

Product Description

Specifications

Product Features

Target Names

Species Detected

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Human Cytokine Array G4000

Human Cytokine Array G4000