The store will not work correctly in the case when cookies are disabled.
JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.
Human FXR/Bile Acid Receptor ELISA Kit RayBio® Human FXR/Bile Acid Receptor ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description Specifications Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit Species Human Protein Name / Synonyms Bile acid receptor (Farnesoid X-activated receptor) (Farnesol receptor HRR-1) (Nuclear receptor subfamily 1 group H member 4) (Retinoid X receptor-interacting protein 14) (RXR-interacting protein 14) Specificity This ELISA antibody pair detects human FXR. Other species not determined. Accession Number Q96RI1 Gene Symbols NR1H4|BAR|FXR|HRR1|RIP14 Gene Id 9971 Quantitative/Semi-Quantitative Quantitative Compatible Sample Types Cell Culture Supernatants, Plasma, Serum Solid Support 96-well Microplate Method Of Detection Colorimetric Design Principle Sandwich-based Sensitivity 2.63 ng/mlNeed more sensitivity? Check out the new IQELISA™ kit for this target . Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
Detection Range 2.63 ng/ml - 700 ng/ml Recommended Dilution (Serum/Plasma) 2 fold Estimated Lead Time 1-2 business days
Risk-Free Guarantee We offer a 100% guarantee on all ELISA Kits or C-Series Membrane Array.
Learn More Amazon Gift Cards! $5 Amazon gift card in every kits purchased.
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Sign up to get promotions on your favorite research tools and research updates.
