Human Gastrin EIA

$376.00
EIA-GAS-1

RayBio® Human/Mouse/Rat Gastrin EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

Introduction

Gastrin is a hormone that stimulates secretion of gastric acid by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the stomach, duodenum, and the pancreas.

Gastrin is a linear peptide that is synthesized as a preprohormone and post-translationally cleaved to form a family of peptides with identical carboxytermini. The predominant circulating form is gastrin-34 (a.k.a. "big gastrin"), but full biologic activity is present in the smaller peptides, i.e., gastrin-17 (a.k.a. "little gastrin") and gastrin-14 (a.k.a. "minigastrin"). In addition, pentagastrin is an artificial 5-amino-acid peptide identical to the carboxyterminus of gastrin. It is worth noting that the five C-terminal amino acids of gastrin and cholecystokinin are identical, which explains their overlapping biological effects.

Gastrin is released in response to certain stimuli including stomach distension, vagal stimulation, hypercalcemia and the presence of partially digested proteins. On the other hand, gastrin release can be inhibited by the presence of gastric acid or inhibitory hormones such as secretin, GIP, VIP, glucagon and calcitonin. As a peptide hormone in GI system, gastrin stimulates gastric acid secretion from parietal cells of the stomach. Additional functions by gastrin include the following, Stimulating parietal cell maturation and fundal growth; Causing chief cells to secrete pepsinogen; Increasing antral muscle mobility and promoting stomach contractions; Strengthening antral contractions against the pylorus, and constricting the pyloric sphincter to slow gastric emptying; inducing pancreatic secretions and gallbladder emptying.


The role of gastrin in certain diseases has also been reported, including Zollinger-Ellison Syndrome, autoimmune gastritis and mucolipidosis. In Zollinger-Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma of the duodenum or the pancreas. In autoimmune gastritis, the immune system attacks the parietal cells leading to hypochlorhydria. This results in an elevated gastrin level in an attempt to compensate for increased pH in the stomach. Eventually, all the parietal cells are lost and achlorhydria results leading to a loss of negative feedback on gastrin secretion. Plasma gastrin concentration is elevated in virtually all individuals with mucolipidosis type IV secondary to a constitutive achlorhydria.

Product Features

  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

The capture antibody provided in this kit recognizes human, mouse and rat Gastrin.

Target Protein Information

Synonyms
Gastrin [Cleaved into: Gastrin (Gastrin component III) (Gastrin-17) (G17) (GAS)]
Accession Number
Gene Names
GAST GAS
Gene ID
2520

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Standard Peptide
  • Assay Diluent(s)
  • Biotinylated Peptide
  • HRP-Streptavidin
  • TMB One-Step Substrate
  • Stop Solution
  • Assay Diagram
  • Positive Control Sample
  • Capture Antibody
  • User Manual
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Orbital shaker
  • Aluminum foil
  • Saran Wrap
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.
  • Storage/Stability

    Standard, Biotinylated Gastrin (GAS) peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

    Antigen Information

    Gene Symbol:
    • GAST

    Product Specifications

    Size:
    1, 2, or 5 x 96-Well Strip Microplate Kit

    Array/ELISA Format

    Species Detected:
    • Human
    • Mouse
    • Rat
    Solid Support:
    96-well Microplate
    Design Principle:
    • Competition-based
    Quantitative/Semi-Quantitative:
    Quantitative
    Method of Detection:
    Colorimetric
    Compatible Sample Types:
    • Serum
    • Cell Culture Supernatants
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    Usage

    This product is furnished for LABORATORY RESEARCH USE ONLY.
    Not for diagnostic or therapeutic use.

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