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25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Human HGF / HGFR Binding Assay Kit

The Human HGF / HGFR Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between HGF and its receptor HGFR (Met) in the presence of potential inhibitors within 4 hours.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	3-5 business days
Species	Human
Compatible Sample Types	Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number	P14210\|P08581
Gene Symbols	HGF, MET
Protein Name / Synonyms	HGF, HGFR (Met)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Research Area	Antibody Drug Development, Protein Therapeutics, Receptor-Ligand Binding
Shipping Type	Blue ice
Storage	4°C

Application Notes

Evaluate inhibitor activity
Screen inhibitors of the Human HGF / HGFR complex
Characterize antibody function
Vaccine development
Selection of patient serum for antibody therapy

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (BAH-MET-HGF.pdf)

SDS (BAH-MET-HGF.pdf)

Application Notes

Evaluate inhibitor activity
Screen inhibitors of the Human HGF / HGFR complex
Characterize antibody function
Vaccine development
Selection of patient serum for antibody therapy

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

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25% off all ELISA kits

25% off all ELISA kits

Human HGF / HGFR Binding Assay Kit