Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

The Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between High affinity immunoglobulin epsilon receptor subunit alpha (FcεRI) and IgE in the presence of potential inhibitors within 4 hours.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	6-8 weeks
Species	Human
Compatible Sample Types	Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number	P12319 / P01854
Gene Id	2205
Gene Symbols	FCER1A, FCE1A / IGHE
Protein Name / Synonyms	High affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha (FcERI); IgE Fc receptor subunit alpha / Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; Ig epsilon chain C region ND
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Shipping Type	Blue ice
Storage	4°C

Product Features

Rapid and sensitive binding affinity characterization
Compatible with various inhibitor types
High throughput screening capability
Simple and easy-to-follow protocol

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
Graduated cylinders: 100 mL and 1 liter
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.

Product Features

Rapid and sensitive binding affinity characterization
Compatible with various inhibitor types
High throughput screening capability
Simple and easy-to-follow protocol

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
Graduated cylinders: 100 mL and 1 liter
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Featured Products

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Featured Products

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

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Reviews

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Featured Products

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit

Human High Affinity Immunoglobulin epsilon Receptor Subunit alpha (FcεRI) / IgE Ligand Binding Assay Kit