Recombinant Human hSIRT1, HEK293 Lysate

Recombinant human hSIRT1 overexpression cell lysate, derived from transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.

$146.01  
230-10073-100
+

Product Specifications

Size:
100 ug

Source

Gene Symbols:
  • SIRT1
Species:
Human
Accession Number:
  • Q96EB6
Gene ID:
  • 23411
Expressed Region:
Ile2-Ser555
Protein Name & Synonyms:
NAD-dependent protein deacetylase sirtuin-1, hSIRT1

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal 8x histidine tag
Purification:
Unpurified overexpression cell lysate
Molecular Weight (kDa):
63.3

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (RayBiotech Cat#: 68QT-BCAPro-S1000) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Specifications

Size
100 ug

Stability & Storage

Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.