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CODE | Sample Type | Number of Plates | Price | ||
---|---|---|---|---|---|
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ELH-IL6-CL-1
|
$420.00 | |||
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ELH-IL6-1
|
$420.00 | |||
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ELH-IL6-CL-2
|
$756.00 | |||
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ELH-IL6-5
|
$1,680.00 | |||
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ELH-IL6-CL-5
|
$1,680.00 | |||
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ELH-IL6-2
|
$756.00 |
Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.
The IL-6 ELISA employs an antibody specific for human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL- 6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Interleukin 6 (IL-6) is a pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and plays an important role in the immune response and acute phase reactions. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate an immune response, e.g., during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6's role as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1R alpha and IL-10.
Sample Type | Average % Recovery | Range (%) |
---|---|---|
Serum | 92.63 | 83-103 |
Plasma | 93.74 | 84-104 |
Cell culture media | 95.65 | 83-105 |
Serum | Plasma | Cell Culture Media | ||
---|---|---|---|---|
1:2 | Average % of expected Range (%) | 95 84-103 | 96 85-105 | 97 84-105 |
1:4 | Average % of expected Range (%) | 96 83-105 | 95 84-103 | 97 85-105 |
Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.