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Human IL-6 ELISA

RayBio® Human IL-6 ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELH-IL6-1
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Antigen Information

Accession Number:
  • P05231
Gene ID:
  • 3569
Gene Symbols:
  • IFNB2
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2)
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Sample Type Average % Recovery Range (%)
Serum 92.63 83-103
Plasma 93.74 84-104
Cell culture media 95.65 83-105

Linearity Results

    Serum Plasma Cell Culture Media
1:2 Average % of expected
Range (%)		 95
84-103		 96
85-105		 97
84-105
1:4 Average % of expected
Range (%)		 96
83-105		 95
84-103		 97
85-105

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Siqi Feng, Ya Su, Li Luo, Jing Fengchuan, Qijian Yi, Serum levels of C1q/tumor necrosis factor related protein-1 in children with Kawasaki disease, Pediatric Research accepted article preview 23 January 2018; doi: 10.1038/pr.2018.16.
    Species: Human
    Sample type: Serum
  2. Xia, Yiru, et al. "mTOR inhibition rejuvenates the aging gingival fibroblasts through alleviating oxidative stress."?Oxidative medicine and cellular longevity?2017 (2017).
    Species: Human
    Sample type: Conditioned Media
  3. Chen, Xia, et al. "Lanthanum chloride inhibits LPS mediated expressions of pro-inflammatory cytokines and adhesion molecules in HUVECs: involvement of NF-?B-Jmjd3 signaling."?Cellular Physiology and Biochemistry?42.5 (2017): 1713-1724.
    Species: Human
    Sample type: Cell culture supernatants
  4. Liu Z., Sun C., Tao R., et al. Pyrroloquinoline Quinone Decelerates Rheumatoid Arthritis Progression by Inhibiting Inflammatory Responses and Joint Destruction via Modulating NF-?B and MAPK Pathways. Inflammation. 2016 Feb;39(1):248-56. doi: 10.1007/s10753-015-0245-7.
    Species: Human
    Sample type: Conditioned Media
  5. Shih K., Janckila A., Lee W., et al. Effects of bariatric weight loss surgery on glucose metabolism, inflammatory cytokines, and serum tartrate-resistant acid phosphatase 5a in obese Chinese adults. Clin Chim Acta. 2016 Jan 30;453:197-202. doi: 10.1016/j.cca.2015.11.004.
    Species: Human
    Sample type: Serum
  6. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  7. Huang CJ., Kwok CF., Chou CH., et al. The effect of exercise on lipid profiles and inflammatory markers in lean male adolescents: a prospective interventional study. J Investig Med. 2015 Jan;63(1):29-34. doi: 10.1097/JIM.0000000000000120.
    Species: Human
    Sample type: Serum
  8. Kuntz S., Asseburg H., Dold S., et al. Inhibition of low-grade inflammation by anthocyanins from grape extract in an in vitro epithelial-endothelial co-culture model. Food Funct. 2015 Apr;6(4):1136-49. doi: 10.1039/c4fo00755g.
    Species: Human
    Sample type: Conditioned Media
  9. Perrigue P., Silva M., Warden C., et al. The histone demethylase jumonji coordinates cellular senescence including secretion of neural stem cell-attracting cytokines. Mol Cancer Res. 2015 Apr;13(4):636-50. doi: 10.1158/1541-7786.MCR-13-0268
    Species: Human
    Sample type: Conditioned Media
  10. Protti A., Fortunato F., Artoni A., et al. Platelet mitochondrial dysfunction in critically ill patients: comparison between sepsis and cardiogenic shock. Crit Care. 2015 Feb 11;19:39. doi: 10.1186/s13054-015-0762-7.
    Species: Human
    Sample type: Plasma
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# Manual (Supernatants / Plasma / Serum)

# Manual (Lysates)

# SDS (Supernatants / Plasma / Serum)

# SDS (Lysates)

# Analysis Tool

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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