RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

Cytometric bead array (CBA) designed to quantitatively measure 6, 9, or 13 human inflammatory factors (see panel options) using flow cytometry. Optimized for serum, plasma, and cell culture supernatant.

Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Protein Name / Synonyms	6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha 9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta, IL-2, IL-12 p70 13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta, IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	6, 9, 13
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Inflammation
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Target Names

6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha

9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70

13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Application Notes

Suggested Applications

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
RayBio^® Serum/Plasma Diluent

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (FAH-INF.pdf)

Datasheet (FAH-INF-1.pdf)

SDS (FAH-INF.pdf)

SDS (FAH-INF-1.pdf)

Excel (2471723f9e55878516e7e1238febe77533f7bbd1.xlsm)

Target Names

6-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha

9-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70

13-plex panel: IFN-gamma, IL-4, IL-6, IL-10, IL-17, TNF alpha, IL-1 beta (IL-1F2), IL-2, IL-12 p70, GCSF, IL-13, IL-23 p19, MCP-1 (CCL2)

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Application Notes

Suggested Applications

Kit Components

RayPlex Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
Lyophilized Protein Standard Mix
Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate
RayBio^® Serum/Plasma Diluent

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Featured Products

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

Product Description

Specifications

Target Names

Product Features

Application Notes

Storage/Stability

Have this product in hand?

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

Product Description

Specifications

Target Names

Product Features

Application Notes

Storage/Stability

Have this product in hand?

Easy to use

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex® Human Inflammation Magnetic Bead Array (13-Plex)

RayPlex® Human Inflammation Magnetic Bead Array (13-Plex)

Easy to use

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Featured Products

Featured Products

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

Product Description

Specifications

Target Names

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

Product Description

Specifications

Related Products

Target Names

Product Features

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Easy to use

Ask A Question

RayPlex® Human Inflammation Bead Array (6,9,13-Plex)

Related Products

RayPlex® Human Inflammation Magnetic Bead Array (13-Plex)

RayPlex® Human Inflammation Magnetic Bead Array (13-Plex)

Easy to use

About Us

Contact Us

Featured Products

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)

RayPlex^® Human Inflammation Bead Array (6,9,13-Plex)