Inhibin B belongs to TGF-beta superfamily, which members also include Inhibin A, TGF-betas (transforming growth factors), activins, BMPs (bone morphogenetic proteins). Among these factors, inhibins are most closely related to activins.
The activin and inhibin are both dimeric protein complexes in structure, and in each complex, the two monomers are linked to one another by a single disulfide bond. In addition, both complexes are derived from the same family of related genes and proteins but differ in their subunit composition. Inhibins are disulfide-linked heterodimers comprising an alpha-subunit and beta-subunit.Inhibin B is composed of one alpha subunit and one beta B subunit; Inhibin A is composed of one alpha subunit and one beta A subunit. By contrast, activins are homo- or hetero-dimers, forming activin A (2 beta A subunits), activin B (2 beta B subunits), or activin AB (1 beta A subunit and 1 beta B subunit).
Inhibins have played important roles in regulating reproductive health. Inhibins and activins generally play opposing roles as modulators of the reproductive axis and have multiple roles in the regulation of reproductive physiology in both females and males. Recently the emerging roles for inhibins in tumorigenesis have been reported. Inhibins has been reported as potential tumor suppressors in several different tissues, including ovary, prostate, endometrium and breasts, with loss of inhibin expression or loss of sensitivity linked to tumor initiation and progression as well as poor patient survival.
The capture antibody provided in this kit recognizes human, mouse and rat Inhibin-B.
Standard, Biotinylated Inhibin B peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.