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    Human Integrin beta 2/CD18 ELISA Kit

    Rating:
    100 % of 100
    1  Review (include details such as sample type used, experimental conditions, etc.)

    RayBio® Human Integrin beta 2/CD18 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

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    As low as $420.00
    In stock
    Only %1 left
    Code:
    ELH-ITGB2
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Protein Name / Synonyms Integrin beta-2 (Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta) (Complement receptor C3 subunit beta) (CD antigen CD18)
    Specificity This ELISA antibody pair detects Human Integrin beta 2. Other species not determined.
    Accession Number P05107
    Gene Symbols CD18|ITGB2|MFI7
    Gene Id 3689
    Quantitative/Semi-Quantitative Quantitative
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 0.22 ng/ml
    Detection Range 0.22 ng/ml - 50 ng/ml
    Recommended Dilution (Serum/Plasma) 8 fold
    Estimated Lead Time 1-2 business days

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    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Reviews

    1. RayBio kit for Human ITGB2
      Rating
      100%
      I recently used the Ray Biotech Human Integrin beta 2 ELISA kit and I am extremely impressed with its performance and reliability. The kit delivered accurate and consistent results throughout my experiments. The sensitivity of the assay was outstanding, allowing to detect even low levels of ITGB2 in my samples. Highly recommended!

      Review by

      from Weill Cornell Medicine,

      on

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