Human Kiloplex Semi-Quantitative Proteomics Array GS

G-Series Human Kiloplex Array. A combination of 25 nonoverlapping arrays. Detects 1000 Human Biomarkers. Suitable for all liquid sample types.

$24,108.70
GSH-CAA-X00-1
+

Antigen Information

Gene Symbols:
ACAN | ACE | ACE2 | ACP5 | ACVR1 | ACVR1B | ACVR2A | ACVR2B | ADA | ADAM12   View more

Assay Format

Number of Targets Detected:
1000
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)
NEED HELP RUNNING THIS ASSAY? 50% Off Discovery Proteomics Services Through december REQUEST QUOTE | MORE INFO

Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

  • High sensitivity (pg/mL to ng/mL levels)
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Test 16 samples on each slide
  • Data analysis software provides one-step computation

Target Names

17-beta-HSD1 (HSD17B1), 2B4 (CD244), 4-1BB (TNFRSF9/CD137), 4-1BB Ligand (TNFSF9), 5'-Nucleotidase (CD73), 6Ckine (CCL21), ACE, ACE-2, Activin A, Activin R1A (ALK-2), Activin R1B (ALK-4), Activin R2A, Activin R2B (ACVR2B), ADA, ADAM-12, ADAM-22, ADAM23, ADAM-8, ADAM-9, ADAMTS-13, ADAMTSL-1, Adiponectin (ACRP30), Adipsin (Complement Factor D), ADP-Ribosyl Cyclase 2 (BST-1/CD157), aFGF (FGF-1), Aggrecan, AgRP, AIF, AKR1C4, Albumin, ALCAM (CD166), ALK-1, ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, AmiGO, AmiGO2, Aminopeptidase LRAP (ERAP2), Aminopeptidase P2 (XPNPEP2), Amnionless, Amphiregulin, AMSH (STAMBP), Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, ANGPTL7, Annexin A5 (Annexin V), APRIL, Arginase 1, ARNT (HIF-1 beta), Artemin, Arylsulfatase A (ARSA), Arylsulfatase B (ARSB), ASAH2 (Neutral Ceramidase), ASAHL, AXL, B3GNT2, B4GalT1, B7-H1 (CD274), B7-H2 (ICOS Ligand), B7-H3 (CD276), B7-H4, BAFF, BAFF R, BAI1, BAMBI (NMA), BATF3, BCAM, BCL-10, BCL-2, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), Beta-glucuronidase (GUSB), beta-NGF, bFGF, BID, Biglycan, BLAME (SLAMF8), BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA (ALK-3), BMPR-II, BOC (Brother of CDO), Bora, Brevican, Brorin (VWC2)...View all 1000 human target proteins

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Detection of Relative Protein Expression
  • Detecting Patterns of Cytokine Expression
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming a Biological Process
Kit Components
  • Antibody Array Slide/s
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • 30 ml Centrifuge Tube
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with samples
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

No posts found

Write A Review
U.S. Non-U.S.

Analysis Tool Bundle Discount

Specify Size
1 x $24,108.70
+
1 x $1,040.30 $312.09

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).
Total list price $25,149.00
Price for all $24,420.79

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Discovery Proteomics Sale Discovery Proteomics Sale

Amazon Gift Card Program

G-Series Testing Service G-Series Testing Service