Human L1000 Array, Membrane

RayBio® L-Series Human Antibody Array 1000 Membrane Kit. Detects 1000 Human Proteins. Suitable for cell culture supernatants, serum, plasma, and tissue lysates.

$1,646.00  
AAH-BLM-1000-2
+

Assay Format

Number of Targets Detected:
1000
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates

Product Specifications

Size:
2 or 4 Sample Kit

Protein Information

Gene Symbols:
A1BG | A2M | ABL1 | ACE | ACE2 | ACKR2 | ACPP | ACVR1 | ACVR1B | ACVR2A   View more

Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

11-beta-HSD1, 2B4, 4-1BB, 6Ckine, A1BG, ACE, ACE-2, ACK1, ACPP, ACTH, Activin A, Activin B, Activin C, Activin R1A, Activin R1B, Activin R2A/B, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, ADAMTSL-2, Adiponectin, Adipsin, Afamin, AgRP, Albumin, ALCAM, Aldolase A, Aldolase B, Aldolase C, ALK, ALK-1, ALK-6, Alpha 1-Acid Glycoprotein, Alpha Lactalbumin, Alpha-1 Microglobulin, Alpha-2 Macroglobulin, Alpha-fetoprotein, Alpha-tropomyosin Protein A, ALPP, AMICA, Aminopeptidase N, Amphiregulin, AMPKa1, Amylin, Amyloid precursor protein, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensin II, Angiotensinogen, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL7, Annexin A7, APC, APCS, APE1, Apelin, Apelin Receptor, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APRIL, Artemin, ASPH, Attractin, AXL, B3GNT1, BACE-1, BAF57, BAFF, BAFF R, BAI1, bax, BCAM, BCMA, BD-1, BDNF, Beta Defensin 4, beta IG-H3, Beta-2 Microglobulin, beta-Catenin, Betacellulin, Beta-endorphin, beta-NGF, bFGF, Biglycan, BIK, BLAME, BLC, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-9, BMPR-IA, BMPR-II, BMX, BNIP2, BNP, BTK, C3a, C5a, CA125, CA15-3, CA19-9, CA9, c-Abl, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, Carboxypeptidase N subunit 2, Cardiotrophin-1, Carnosine Dipeptidase-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CCL28, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD163, CD200, CD23, CD24, CD26, CD27, CD30, CD30 Ligand, CD36, CD38, CD40, CD40 Ligand, CD44, CD45, CD46, CD47, CD55, CD59, CD74, CD79 alpha, CD80, CD90, CD97, CEA, CEACAM-1, Cerberus 1, Ceruloplasmin, CFHR2, Chem R23, Chemerin, CHI3L1, Chordin-Like 2, Chordin-Like-1, Chromogranin-A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLC, CLEC3B, Clusterin, CNTF, CNTF R alpha, Coagulation Factor III, Coagulation Factor X, Coagulation factor XIII A, Coagulation factor XIII B, COCO, Complement component C2, Complement component C7, Complement component C8 beta chain, Complement component C9, Complement Factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-Peptide, Creatinine, CRIM1, Cripto-1, Crossveinless-2, CRP, CRTAM, CRTH-2, Cryptic, CSH1, CSK, CTACK, CTGF, CTLA-4, CutA, CXCL14, CXCL16, CXCL5, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 18, Cytokeratin 19, Cytokeratin 8, D6, DAN, DANCE, DBI, DCBLD2, DcR3, D-Dimer, DDR2, Decorin, Defensin alpha 1, Defensin alpha 1/3, Desmin, DKK-1, Dkk-3, Dkk-4, DLL1, DLL4, DMP-1, DR3, DR6, Dtk, E-Cadherin, EDA-A2, EDAR, EDG-1, EGF, EGFR, EG-VEGF, EMAP-II, Endocan, Endoglin, Endostatin, Endothelin Receptor A, Endothelin-1, ENPP-2, EN-RAGE, Eotaxin-1, Eotaxin-2, Eotaxin-3, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, Epiregulin, ErbB2, ErbB3, ErbB4, ERRa, Erythropoietin, Erythropoietin R, ESAM, E-Selectin, EV15L, Exostosin-like 2, FABP1, FABP2, FABP3, FABP4, FADD, FAK, FAM3B, FAP, Fas, Fas Ligand, Fc gamma RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGF R3, FGF R4, FGF R5, FGF-10, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7, FGF-8, FGF-9, FGF-BP, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, FOLR1, FOXN3, FOXO1, FOXP3, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, FRK, FSH, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas 1, GASP-1, GASP-2, Gastrin, GATA-3, GATA-4, GCP-2, GCSF, GCSF R, GDF-1, GDF-11, GDF-15, GDF-3, GDF-5, GDF-8, GDF-9, GDNF, Gelsolin, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, Ghrelin, GITR, GITR Ligand, GLP-1, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, GMNN, gp130, GPBB, GPI, GPR-39, GPX1, GPX3, Granzyme A, Grb2, Gremlin-1, GRO alpha, GRO alpha/beta/gamma, Growth Hormone, Growth Hormone R, Growth Hormone Variant, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, HB-EGF, HCC-1, HCC-4, hCG alpha, hCG beta, Hck, HCR, HE4, Hemopexin, Hepassocin, Hepcidin, HGF, HGFR, HOXA10, HP, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP70-9B, HSP90, HSPA8, HTRA2, HVEM, I-309, IBSP, ICAM-1, ICAM-2, ICAM-3, ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-rp1, IL-1 alpha, IL-1 beta, IL-1 R1, IL-1 R2, IL-1 R3, IL-1 R4, IL-1 R6, IL-1 R8, IL-1 R9, IL-1 Ra, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-18 BP alpha, IL-18 R alpha, IL-18 R beta, IL-19, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 p19, IL-23 R, IL-24, IL-26, IL-27, IL-27 R alpha, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-33, IL-34, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-36 Ra, IL-37, IL-38, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IMP1, INSRR, Insulin, Insulin R, Insulysin, Integrin alpha-5, Integrin alpha-M, Integrin beta-3, IP-10, I-TAC, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, Kininogen, KLF4, Kremen-1, Kremen-2, Lactoferrin, LAG-3, Layilin, LBP, LCK, LDL R, LECT2, Lefty-A, Legumain, Leptin, Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT, LIMPII, LIN41, Lipocalin-1, Lipocalin-2, Livin, LOX-1, LPS, LRG1, LRP-1 Cluster II, LRP-6, L-Selectin, LTBP1, LTK, Lumican, Luteinizing hormone, Lymphotactin, Lymphotoxin beta, Lymphotoxin beta R, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, MCP-1, MCP-3, MCP-4, M-CSF, M-CSF R, MDC, Mer, Mesothelin, MFG-E8, MFRP, MICA, MICB, Midkine, MIF, MIG, MINA, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1, MSHa, MSP alpha, MTUS1, MuSK, Myoglobin, NAIP, Nanog, NAP-2, NCAM-1, NELL2, Neprilysin, Nesfatin-1, Nestin, NET1, Netrin G2, Netrin-1, Netrin-3, Netrin-4, Neuritin, NeuroD1, Neurokinin-A, Neuropeptide Y, Neuropilin-2, Neurturin, NF1, NGFR, Nidogen-1, NM23-H1/H2, Notch-1, NOV, NPTX1, NPTXR, Nr-CAM, NRG1 Isoform GGF2, NRG1-alpha, NRG1-beta 1, NRG2, NRG3, NSE, NT-3, NT-4, OCT-4, Omentin, Oncostatin M, Orexin-A, Orexin-B, Osteoactivin, Osteocalcin, Osteocrin, Osteopontin, Osteoprotegerin, OX40, OX40 Ligand, p21, p27/Kip1, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, PAPP-A, PARC, Park7, P-Cadherin, PCAF, PD-1, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PDX-1, PECAM-1, Pentraxin-3, Pepsinogen 1, Pepsinogen 2, Peroxiredoxin-6, Persephin, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, Platelet Factor 4, Pleiotrophin, PLGF, PLUNC, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, PR Isoform B, Pref-1, Presenilin 1, Presenilin 2, Pro Relaxin-3, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, Proglucagon, Progranulin, Prohibitin, Prolactin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, P-Selectin, PSP94, PTH, PTHLP, PTPRD, PYK2, PYY, RAGE, RANK, RANTES, Ras, RBP4, RECK, RELM alpha, RELM beta, RELT, Resistin, Ret, RIP1, ROBO4, ROCK1, ROCK2, ROR1, ROR2, ROS1, Ryk, S100 A10, S100 A4, S100 A6, S100 A8, S100 A8/A9, S100 B, SAA, SART1, SART3, SCF, SCF R, SCG3, SDF-1, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A9, Serpin B5, Serpin D1, Serpin F1, Serpin G1, Serpin I1, SERTAD2, sFRP-1, sFRP-3, sFRP-4, SHBG, SIGIRR, Siglec-5, Siglec-9, SLPI, SMAC, SMAD1, SMAD4, SMAD5, SMAD7, SMAD9, SNCG, Soggy-1, Somatostatin R5, Sonic Hedgehog N-Terminal, SOST, SOX17, SOX2, SPARC, SPARCL1, Spinesin, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TACI, TAF4, TARC, TEC, TECK, TFF1, TFF3, TFPI, TfR, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R2, TGF beta R3, TGF-beta 5, TGF-beta RI, Thrombin, Thrombomodulin, Thrombopoietin, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymidine Kinase-1, Thymopoietin isform alpha, Thyroglobulin, Thyroid Peroxidase, Tie-1, Tie-2, TIM-1, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF1, TMEFF2, TNF alpha, TNF beta, TNF RI, TNF RII, TNK1, TOPORS, TPA, TRA-1-60, TRA-1-81, TRADD, TRAIL, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, TRANCE, Transferrin, Trappin-2, TREM-1, TRKB, TROP1, Troponin C, Troponin I, Troponin T, TROY, TRPC1, TRPC6, TRPM7, Trypsin 1, TSG-6, TSH, TSLP, TSLP R, TWEAK, TWEAK R, TXK, TYK2, Ubiquitin+1, uPA, uPAR, Uromodulin, Vasopressin, Vasorin, VCAM-1, VDUP-1, VE-Cadherin, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-binding protein, Vitamin K-dependent protein S, Vitronectin, VWF, WIF-1, Wilms Tumor 1, WISP-1, XEDAR, XIAP, ZAG, ZAP70

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Detection Buffer C
  • Detection Buffer D
  • Wash Buffer 1
  • Wash Buffer 2
  • HRP-Conjugated Streptavidin Concentrate
  • Plastic Sheets
  • Spin Columns
  • Labeling Buffer
  • Lysis Buffer
  • Plastic Incubation Tray
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • 2?5 ml tube
  • 15 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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