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Human L1000 Array, Membrane

RayBio® L-Series Human Antibody Array 1000 Membrane Kit. Detects 1000 Human Proteins. Suitable for cell culture supernatants (A) and serum, plasma and tissue lysates (B).

Cell Culture Media Manual Serum, Plasma, Lysate Manual

$1,939.20  
AAH-BLM-1000A-2
+

Antigen Information

Gene Symbols:
A1BG | A2M | ABL1 | ACE | ACE2 | ACKR2 | ACPP | ACVR1 | ACVR1B | ACVR2A   View more

Assay Format

Number of Targets Detected:
1000
Species Detected:
  • Human

Product Specifications

Size:
2 or 4 Sample Kit

Product Feature

  • High density arrays
  • 1000 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Name

11-beta-HSD1, 2B4, 4-1BB, 6Ckine, A1BG, ACE, ACE-2, ACK1, ACPP, ACTH, Activin A, Activin B, Activin C, Activin R1A, Activin R1B, Activin R2A/B, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4, ADAMTS-5, ADAMTSL-2, Adiponectin, Adipsin, Afamin, AgRP, Albumin, ALCAM, Aldolase A, Aldolase B, Aldolase C, ALK, ALK-1, ALK-6, Alpha 1-Acid Glycoprotein, Alpha Lactalbumin, Alpha-1 Microglobulin, Alpha-2 Macroglobulin, Alpha-fetoprotein, Alpha-tropomyosin Protein A, ALPP, AMICA, Aminopeptidase N, Amphiregulin, AMPKa1, Amylin, Amyloid precursor protein, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensin II, Angiotensinogen, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL7, Annexin A7, APC, APCS, APE1, Apelin, Apelin Receptor, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APRIL, Artemin, ASPH, Attractin, AXL, B3GNT1, BACE-1, BAF57, BAFF, BAFF R, BAI1, bax, BCAM, BCMA, BD-1, BDNF, Beta Defensin 4, beta IG-H3, Beta-2 Microglobulin, beta-Catenin, Betacellulin, Beta-endorphin, beta-NGF, bFGF, Biglycan, BIK, BLAME, BLC, BMP-15, BMP-2, BMP-3, BMP-3b, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-9, BMPR-IA, BMPR-II, BMX, BNIP2, BNP, BTK, C3a, C5a, CA125, CA15-3, CA19-9, CA9, c-Abl, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, Carboxypeptidase N subunit 2, Cardiotrophin-1, Carnosine Dipeptidase-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CCL28, CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD163, CD200, CD23, CD24, CD26, CD27, CD30, CD30 Ligand, CD36, CD38, CD40, CD40 Ligand, CD44, CD45, CD46, CD47, CD55, CD59, CD74, CD79 alpha, CD80, CD90, CD97, CEA, CEACAM-1, Cerberus 1, Ceruloplasmin, CFHR2, Chem R23, Chemerin, CHI3L1, Chordin-Like 2, Chordin-Like-1, Chromogranin-A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLC, CLEC3B, Clusterin, CNTF, CNTF R alpha, Coagulation Factor III, Coagulation Factor X, Coagulation factor XIII A, Coagulation factor XIII B, COCO, Complement component C2, Complement component C7, Complement component C8 beta chain, Complement component C9, Complement Factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-Peptide, Creatinine, CRIM1, Cripto-1, Crossveinless-2, CRP, CRTAM, CRTH-2, Cryptic, CSH1, CSK, CTACK, CTGF, CTLA-4, CutA, CXCL14, CXCL16, CXCL5, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 18, Cytokeratin 19, Cytokeratin 8, D6, DAN, DANCE, DBI, DCBLD2, DcR3, D-Dimer, DDR2, Decorin, Defensin alpha 1, Defensin alpha 1/3, Desmin, DKK-1, Dkk-3, Dkk-4, DLL1, DLL4, DMP-1, DR3, DR6, Dtk, E-Cadherin, EDA-A2, EDAR, EDG-1, EGF, EGFR, EG-VEGF, EMAP-II, Endocan, Endoglin, Endostatin, Endothelin Receptor A, Endothelin-1, ENPP-2, EN-RAGE, Eotaxin-1, Eotaxin-2, Eotaxin-3, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, Epiregulin, ErbB2, ErbB3, ErbB4, ERRa, Erythropoietin, Erythropoietin R, ESAM, E-Selectin, EV15L, Exostosin-like 2, FABP1, FABP2, FABP3, FABP4, FADD, FAK, FAM3B, FAP, Fas, Fas Ligand, Fc gamma RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGF R3, FGF R4, FGF R5, FGF-10, FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FGF-4, FGF-5, FGF-6, FGF-7, FGF-8, FGF-9, FGF-BP, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, FOLR1, FOXN3, FOXO1, FOXP3, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, FRK, FSH, Furin, FYN, GADD45A, Galanin, Galectin-1, Galectin-3, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas 1, GASP-1, GASP-2, Gastrin, GATA-3, GATA-4, GCP-2, GCSF, GCSF R, GDF-1, GDF-11, GDF-15, GDF-3, GDF-5, GDF-8, GDF-9, GDNF, Gelsolin, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, Ghrelin, GITR, GITR Ligand, GLP-1, Glucagon, Glut1, Glut2, Glut3, Glut5, Glyoxalase 1, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, GMNN, gp130, GPBB, GPI, GPR-39, GPX1, GPX3, Granzyme A, Grb2, Gremlin-1, GRO alpha, GRO alpha/beta/gamma, Growth Hormone, Growth Hormone R, Growth Hormone Variant, GRP, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, HB-EGF, HCC-1, HCC-4, hCG alpha, hCG beta, Hck, HCR, HE4, Hemopexin, Hepassocin, Hepcidin, HGF, HGFR, HOXA10, HP, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP70-9B, HSP90, HSPA8, HTRA2, HVEM, I-309, IBSP, ICAM-1, ICAM-2, ICAM-3, ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-rp1, IL-1 alpha, IL-1 beta, IL-1 R1, IL-1 R2, IL-1 R3, IL-1 R4, IL-1 R6, IL-1 R8, IL-1 R9, IL-1 Ra, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E, IL-17F, IL-18 BP alpha, IL-18 R alpha, IL-18 R beta, IL-19, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 p19, IL-23 R, IL-24, IL-26, IL-27, IL-27 R alpha, IL-28A, IL-29, IL-3, IL-3 R alpha, IL-31, IL-31 RA, IL-33, IL-34, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-36 Ra, IL-37, IL-38, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IMP1, INSRR, Insulin, Insulin R, Insulysin, Integrin alpha-5, Integrin alpha-M, Integrin beta-3, IP-10, I-TAC, ITK, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, Kininogen, KLF4, Kremen-1, Kremen-2, Lactoferrin, LAG-3, Layilin, LBP, LCK, LDL R, LECT2, Lefty-A, Legumain, Leptin, Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT, LIMPII, LIN41, Lipocalin-1, Lipocalin-2, Livin, LOX-1, LPS, LRG1, LRP-1 Cluster II, LRP-6, L-Selectin, LTBP1, LTK, Lumican, Luteinizing hormone, Lymphotactin, Lymphotoxin beta, Lymphotoxin beta R, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, MCP-1, MCP-3, MCP-4, M-CSF, M-CSF R, MDC, Mer, Mesothelin, MFG-E8, MFRP, MICA, MICB, Midkine, MIF, MIG, MINA, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-14, MMP-15, MMP-16, MMP-19, MMP-2, MMP-20, MMP-24, MMP-25, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1, MSHa, MSP alpha, MTUS1, MuSK, Myoglobin, NAIP, Nanog, NAP-2, NCAM-1, NELL2, Neprilysin, Nesfatin-1, Nestin, NET1, Netrin G2, Netrin-1, Netrin-3, Netrin-4, Neuritin, NeuroD1, Neurokinin-A, Neuropeptide Y, Neuropilin-2, Neurturin, NF1, NGFR, Nidogen-1, NM23-H1/H2, Notch-1, NOV, NPTX1, NPTXR, Nr-CAM, NRG1 Isoform GGF2, NRG1-alpha, NRG1-beta 1, NRG2, NRG3, NSE, NT-3, NT-4, OCT-4, Omentin, Oncostatin M, Orexin-A, Orexin-B, Osteoactivin, Osteocalcin, Osteocrin, Osteopontin, Osteoprotegerin, OX40, OX40 Ligand, p21, p27/Kip1, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, PAPP-A, PARC, Park7, P-Cadherin, PCAF, PD-1, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PDX-1, PECAM-1, Pentraxin-3, Pepsinogen 1, Pepsinogen 2, Peroxiredoxin-6, Persephin, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, Platelet Factor 4, Pleiotrophin, PLGF, PLUNC, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, PR Isoform B, Pref-1, Presenilin 1, Presenilin 2, Pro Relaxin-3, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, Proglucagon, Progranulin, Prohibitin, Prolactin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-free, PSA-total, P-Selectin, PSP94, PTH, PTHLP, PTPRD, PYK2, PYY, RAGE, RANK, RANTES, Ras, RBP4, RECK, RELM alpha, RELM beta, RELT, Resistin, Ret, RIP1, ROBO4, ROCK1, ROCK2, ROR1, ROR2, ROS1, Ryk, S100 A10, S100 A4, S100 A6, S100 A8, S100 A8/A9, S100 B, SAA, SART1, SART3, SCF, SCF R, SCG3, SDF-1, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A9, Serpin B5, Serpin D1, Serpin F1, Serpin G1, Serpin I1, SERTAD2, sFRP-1, sFRP-3, sFRP-4, SHBG, SIGIRR, Siglec-5, Siglec-9, SLPI, SMAC, SMAD1, SMAD4, SMAD5, SMAD7, SMAD9, SNCG, Soggy-1, Somatostatin R5, Sonic Hedgehog N-Terminal, SOST, SOX17, SOX2, SPARC, SPARCL1, Spinesin, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TACI, TAF4, TARC, TEC, TECK, TFF1, TFF3, TFPI, TfR, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R2, TGF beta R3, TGF-beta 5, TGF-beta RI, Thrombin, Thrombomodulin, Thrombopoietin, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymidine Kinase-1, Thymopoietin isform alpha, Thyroglobulin, Thyroid Peroxidase, Tie-1, Tie-2, TIM-1, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A, TLR1, TLR2, TLR3, TLR4, TMEFF1, TMEFF2, TNF alpha, TNF beta, TNF RI, TNF RII, TNK1, TOPORS, TPA, TRA-1-60, TRA-1-81, TRADD, TRAIL, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, TRANCE, Transferrin, Trappin-2, TREM-1, TRKB, TROP1, Troponin C, Troponin I, Troponin T, TROY, TRPC1, TRPC6, TRPM7, Trypsin 1, TSG-6, TSH, TSLP, TSLP R, TWEAK, TWEAK R, TXK, TYK2, Ubiquitin+1, uPA, uPAR, Uromodulin, Vasopressin, Vasorin, VCAM-1, VDUP-1, VE-Cadherin, VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-binding protein, Vitamin K-dependent protein S, Vitronectin, VWF, WIF-1, Wilms Tumor 1, WISP-1, XEDAR, XIAP, ZAG, ZAP70

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human 507 and 493 Antibody Array Membranes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • HRP-Conjugated Streptavidin Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • Plastic Sheets
  • Floating Dialysis Rack
  • Dialysis Vials
  • Wash Buffer 1
  • Wash Buffer 2
  • Spin Columns
  • Plastic Incubation Tray
  • (Lysate specific kit) Lysis Buffer
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • Pipettors, pipette tips and other common lab consumables
  • 2~5 ml tube
  • 50 ml conical collection tube
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • A chemiluminescent blot documentation system (such as UVPTMs ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated Samples in PBS
  4. Block array membranes
  5. Incubate with Samples
  6. Incubate with HRP-Conjugated Streptavidin
  7. Incubate with Detection Buffers
  8. Image with chemiluminescent imaging system
  9. Perform densitometry and analysis

Storage/Stability

For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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