Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

TheHuman MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit uses a rapid, simple, and sensitive method to characterize the binding affinity between MAdCAM1 and Integrin alpha 4 beta 7 in the presence of potential inhibitors within 4 hours.

$931.00

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	6-8 weeks
Species	Human
Compatible Sample Types	Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides
Accession Number	Q13477 / P13612, P26010
Gene Id	8174 / 3676, 3695
Gene Symbols	MADCAM1 / ITGA4 CD49D, ITGB7
Protein Name / Synonyms	Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) (hMAdCAM-1) / Integrin alpha-4 (CD49 antigen-like family member D) (Integrin alpha-IV) (VLA-4 subunit alpha) (CD antigen CD49d), Integrin beta-7 (Gut homing receptor beta subunit)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Research Area	Cell Adhesion, Receptor-Ligand Binding
Shipping Type	Blue ice
Storage	4°C

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

Storage/Stability

The kit may be stored at 4°C up to 1 month from the date of shipment. Shelf life can be extended up to 6 months by storing protein and antibodies, at -20°C immediately upon delivery. All other kit components should be stored at 4°C.

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (BA-PRELIM.pdf)

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Assay Diluent
Ligand Protein
Detection Antibody
HRP Conjugated Anti-IgG
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Shaker
Pipettes capable of accurately delivering 2 µL to 1 mL volumes
Pipettes capable of delivering 1-25 mL volumes for reagent preparation
100 mL and 1 liter graduated cylinders
Distilled or deionized water
Tubes to prepare sample dilutions

Assay Procedure Summary

Prepare all reagents and test reagent samples as instructed.
Add 100 µL test reagent samples to each well. Incubate 2.5 hours at room temperature or overnight at 4°C.
Add 100 µL the prepared Detection Antibody solution to each well. Incubate 1 hour.
Add 100 µL the prepared HRP Conjugated Anti-IgG solution to each well. Incubate 1 hour.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes.
Add 50 µL Stop Solution directly to each well. Read at 450 nm immediately.

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Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Featured Products

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit

Product Description

Specifications

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Human MAdCAM1 / Integrin alpha 4 beta 7 Ligand Binding Assay Kit