The RayBio® Human MANF ELISA kit for Serum and Plasma samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Sensitivity | 0.013 ng/ml |
| Detection Range | 0.013 ng/ml - 5 ng/ml |
| Specificity | This ELISA antibody pair detects human MANF. Other species not determined. |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2-10 fold |
| Accession Number | P55145 |
| Gene Id | 7873 |
| Gene Symbols | MANF |
| Protein Name / Synonyms | Mesencephalic astrocyte-derived neurotrophic factor (Arginine-rich protein) (Protein ARMET) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| Serum | 93.74 | 70-119 |
| Plasma | 94.33 | 72-138 |
| Cell culture media | 119.6 | 94-134 |
Linearity Results
| Sample Type | Serum | Plasma | Cell Culture Media | |
|---|---|---|---|---|
| 1:2 | Average % of Expected | 110.9 | 102.4 | 103.1 |
| Range (%) | 108-114 | 84-115 | 98-108 | |
| 1:4 | Average % of Expected | 129.5 | 110.9 | 108.7 |
| Range (%) | 127-132 | 84-134 | 107-111 | |
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard and sample dilutions
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.