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Custom Human/Mouse Cleaved-Caspase-7 (D198) Cell-based ELISA

RayBio®Human/mouse cleaved CASP-7 (D198) Cell-based ELISA Kit. This ELISA is for measuring cleaved CASP-7 (Asp-198) in human and mouse adherent cell lines.
Lead time 4-6 weeks

Old price: $633.00
$506.40
You save: $126.60 (20%)
CBEL-CASP7-D198-1
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Antigen Information

Accession Number:
  • P55210
Gene ID:
  • 840
Gene Symbols:
  • CASP7
  • MCH3
Protein Name & Synonyms:
Caspase-7 (CASP-7) (EC 3.4.22.60) (Apoptotic protease Mch-3) (CMH-1) (ICE-like apoptotic protease 3) (ICE-LAP3) [Cleaved into: Caspase-7 subunit p20 Caspase-7 subunit p11]
Target Species:
  • Human
  • Mouse

Assay Format

Specificity:
The antibodies provided in this kit recognizes human/mouse cleaved-caspase-7 cleaved at site Aspartic Acid-198

Product Features

  • Rapidly measure cleaved protein in adherent cell lines
  • Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
  • No sample lysis is needed
  • Compatible with a standard ELISA plate reader

Application Notes

Kit Components
  • Uncoated 96-well Strip Microplate
  • Wash Buffers
  • Fixing Solution
  • Quneching Buffer
  • Blocking Buffer
  • Anti-cleaved Antibody
  • Anti-pan antibody
  • HRP-Conjugated Secondary Antibody
  • TMB One-Step Substrate
  • Stop Solution
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Seed 10,000-30,000 cells into each well and incubate overnight.
  2. Apply various treatment, inhibitors or activators according to manufacturer's instructions.
  3. Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
  4. Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
  5. Add 200 µl of Blocking Solution and incubate for 1 h at 37°C.
  6. Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
  7. Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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