RayBio® Human/Mouse/Rat Neuromedin-B EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse, Rat |
| Sensitivity | 0.3 ng/ml |
| Detection Range | 0.1-1,000 ng/ml |
| Specificity | This EIA kit is designed to detect human, mouse, and rat Neuromedin-B-32 |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | Human: 2x / Mouse: 2x / Rat: 2x |
| Accession Number | P08949 |
| Gene Id | 4828 |
| Gene Symbols | NMB |
| Protein Name / Synonyms | Neuromedin-B [Cleaved into: Neuromedin-B- |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Competition-based |
| Method Of Detection | Colorimetric |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Neuromedin B (NMB) is one of the bombesin (BN)-related peptides in mammals. It was originally purified from pig spinal cord, and mainly presents in human central nervous system and gastrointestinal tract. Neuromedin regulates various physiological functions, including exocrine and endocrine secretions, smooth muscle contraction, feeding, blood pressure, blood glucose, body temperature and cell growth. NMB binds to its receptor, a G-protein coupled receptor, on the cell surface and triggers several intracellular signaling cascades including phospholipase activation, calcium mobilization and protein kinase C (PKC) activation.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.