Human NF-κB Array C2

RayBio® C-Series Human NF-kB Array 2 Kit. Detects 34 proteins in human samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

$440.36  
AAH-NFKB-2-2
+

Antigen Information

Gene Symbols:
ASC | BCL10 | BIRC2 | BIRC3 | CD40 | CIS4 | FADD | FAS | FIP3 | IKBB   View more

Assay Format

Pathway:
  • NFkB Signaling
Number of Targets Detected:
34
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ASC
(PYCARD)
BCL-10
CD40
(TNFRSF5)
cIAP-1
(BIRC2/hIAP-2)
cIAP-2
c-Rel
FADD
Fas
(TNFRSF6/Apo-1)
IkB-alpha
IKB-b
(NFKBIB)
IkB-epsilon
(NF-kappa-BIE)
IKK-B
IKK-E
(IKBKE)
IKK-gamma
(NEMO/FIP-3)
IL-1 Ra
(IL-1 F3)
IL-1 R1
IL-17 RA
IL-1 R5
(IL-18 R alpha)
IRAK1
IRF5
JNK2
(MAPK9 isoform alpha-1)
Lymphotoxin beta R
(TNF RIII)
MyD88
NFKB1
nfkb2
NGFR
(TNFRSF16)
p53
socs6
STING
  • Gene Symbol: TMEM173 ERIS MITA STING
  • Gene ID: 340061
  • Accession #: Q86WV6
  • ELISA Kit: RayBio
TLR2
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TRAIL R1
(TNFRSF10A/DR4)
TRAIL R2
(TNFRSF10B/DR5)
 

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human NF-kB Array C2 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • Plastic Sheets
  • Array Map Template
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service