Nucleobindin-2 has roles in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. Nucleobindin-2 binds calcium and has EF-folding domains. Nucleobindin-2 is further cleaved into a 82 amino acids peptide, Nesfatin, which plays important roles regulating food intake, energy homeostasis, and blood pressure.

Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.