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RayBio® Human PRND ELISA Kit.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species
Human
Targets Detected
PRND
Protein Name / Synonyms
Prion-like protein doppel (PrPLP) (Prion protein 2)
Specificity
This ELISA antibody pair detects human PRND. Other species not determined.
Accession Number
Q9UKY0
Quantitative/Semi-Quantitative
Quantitative
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Sensitivity
0.06 ng/ml
Detection Range
0.06 ng/ml - 10 ng/ml
Recommended Dilution (Serum/Plasma)
2 fold
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We offer a 100% guarantee on all ELISA Kits or C-Series Membrane Array.
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Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data Kit Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate Other Materials Required
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately. Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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