RayBio® Human/Mouse/Rat Pro-Neuropeptide Y/CPON EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human, Mouse, Rat |
| Sensitivity | 0.19 ng/ml or 16.59 pM |
| Detection Range | 0.1-1,000 ng/ml |
| Specificity | This kit was designed to recognize the C-terminus of pro-NPY and therefore will detect full-length pro-NPY and CPON. However, it does not recognize active forms including NPY 1-36, 2-36, 3-36 or 3-35. This kit detects both CPON and the 69 aa pro-form of NPY. Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II and APC. |
| Compatible Sample Types | Plasma, Serum |
| Accession Number | P01303 |
| Gene Id | 4852 |
| Gene Symbols | NPY |
| Protein Name / Synonyms | Pro-neuropeptide Y [Cleaved into: Neuropeptide Y (Neuropeptide tyrosine) (NPY) C-flanking peptide of NPY (CPON)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Competition-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Neuropeptide Y (NPY) is a 36 amino acid peptide hormone found in the neural system and has an important role in obesity.
The main effect of Neuropeptide Y is increased food intake and decreased physical activity. It also increases the proportion of energy stored as fat and blocks nociceptive signals to the brain. In addition to its role in obesity, Neuropeptide Y has been associated with a number of other physiologic processes in the brain, including the regulation of energy balance, memory and learning, and epilepsy.
Animal studies strongly demonstrate that the stimulation of Neuropeptide Yergic activity via the administration of certain Neuropeptide Y agonists increases food intake compared to control animals. The effects of Neuropeptide Yergic activity on food intake is also demonstrated by the blockade of certain Neuropeptide Y receptors (Y1 and Y5 receptors), which expectedly inhibited Neuropeptide Yergic activity; thus, decreases food intake. For its role in obesity, an increase in Neuropeptide Y is caused by high levels of glucocorticosteriods through directly activating type II glucocorticosteriods receptors and indirectly, by abolishing the negative feedback of CRF on Neuropeptide Y synthesis and release. Meanwhile, obesity-induced insulin resistance and the mutation of the leptin receptor (ObRb) results in the abolishment of other negative feedback mechanisms to regulate Neuropeptide Yergic activity and ultimately food intake. High levels of Neuropeptide Y were also found in the cerebrospinal fluid of patients with anorexia nervosa.
The 291 bp coding sequence of the NPY gene synthesizes the pre-pro form of NPY, a 97 aa precursor peptide (see diagram below). Cleavage of the n-terminal 29 aa results in pro-NPY (69 aa), which then undergoes a further cleavage, yielding NPY 1-39 along with the 30-aa C-terminal peptide known as C-flanking peptide of NPY (CPON). NPY 1-39 is further processed to the mature form (NPY 1-36). The function of CPON is not currently understood.
The main effect of Neuropeptide Y is increased food intake and decreased physical activity. It also increases the proportion of energy stored as fat and blocks nociceptive signals to the brain. In addition to its role in obesity, Neuropeptide Y has been associated with a number of other physiologic processes in the brain, including the regulation of energy balance, memory and learning, and epilepsy.
Animal studies strongly demonstrate that the stimulation of Neuropeptide Yergic activity via the administration of certain Neuropeptide Y agonists increases food intake compared to control animals. The effects of Neuropeptide Yergic activity on food intake is also demonstrated by the blockade of certain Neuropeptide Y receptors (Y1 and Y5 receptors), which expectedly inhibited Neuropeptide Yergic activity; thus, decreases food intake. For its role in obesity, an increase in Neuropeptide Y is caused by high levels of glucocorticosteriods through directly activating type II glucocorticosteriods receptors and indirectly, by abolishing the negative feedback of CRF on Neuropeptide Y synthesis and release. Meanwhile, obesity-induced insulin resistance and the mutation of the leptin receptor (ObRb) results in the abolishment of other negative feedback mechanisms to regulate Neuropeptide Yergic activity and ultimately food intake. High levels of Neuropeptide Y were also found in the cerebrospinal fluid of patients with anorexia nervosa.
The 291 bp coding sequence of the NPY gene synthesizes the pre-pro form of NPY, a 97 aa precursor peptide (see diagram below). Cleavage of the n-terminal 29 aa results in pro-NPY (69 aa), which then undergoes a further cleavage, yielding NPY 1-39 along with the 30-aa C-terminal peptide known as C-flanking peptide of NPY (CPON). NPY 1-39 is further processed to the mature form (NPY 1-36). The function of CPON is not currently understood.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Pro-NPY Standard, Biotinylated Pro-NPY peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.