Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
HSPA1A, HTRA1, Human Agrin, IBP160 , IDH1, IDH3A, IFRD1 , IMP2, IGFBP-rp1, IGSF4B, Ihh, ILK, Inhibin beta, Integrin b1, Integrin beta 6, Intergrin a6, IQGAP1 , IQGAP2 , IRE1, IRS2, ISOC2, ITGB4BP, ITIH1, ITIH2, ITIH3, ITIH4 a, JAM-A, JARID2, Karyopherin beta 1, Keratin 36, Keratin 38, KHSRP, KIAA0319L, KIAA1468, KIAA1967, KIF5B , Kilon, KLK-B1, KMD4B, KMT2B, KRT31, KRT72, KRT73, KRT82, KRT85 N-terminal, KRTDAP, KRTHA3B, KSR1, LAD, LAF4, LAIR1, LAM b1, LAMA, Lamin A/C, Lamin B1, Lamin B2, Laminin 2 alpha, Laminin b2, Laminin gamma 1, LAMP, LAMP1, LAMP2, LAP3, LASP1, Latent TGF beta bp2, LCAT, LCMT2, LDHA, LDHB, LEDGF, LEKTI, LILRA3, LIMS1, LMAN2, LMW-PTP, LOK, LOX, LOXL1, LRP 4, LTA4H, LTBP4, Lubricin, LUZP1, LYPA1, Lysozyme, MAGI2, MAGP-2, MAN1, MANF, Mannosidase II, MAP1A, MAPRE1, MARCKS, Complement MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MCM5 , MCMP2, MDH1, MDH2, ME1, Meprin alpha, Metallothionein, Metavinculin, MFAP4, MFI2, mGLUR5, MGP, Mimecan, MINPP1, MLCK, MMR, MMRN1, MN1, Moesin, MP1, MPCA, Myeloperoxidase, MRP 1, MSH6, mTOR, MUCDHL, Multimerin 2, MyBPC3, MYH2, MYH6, MYH7, MYHC, MYL12B , MYL3, MYO5A, Myoferlin , Myosin 18B, Myotrophin, NABC1, NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin, Nectin-1, Nectin-3, NEDD8, Neogenin, Nesprin2, Neurabin 1, N-Cadherin, Neurofibromin, Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, Nidogen-2, NIT2, NME3, nNOS, Noelin, Non-muscle Actin, Non-muscle Myosin IIA, Notch-2, Notch-2 ICD, NPAS3, NPM1, NQO2, NT5C3, Nucleobindin 1, NUP98, OBCAM, OIT3, Olfactomedin-2, Ornithine Carbamoyltransferase , Orosomucoid 2, ORP150, OSBP1, OSCAR, Oncostatin M R beta, Osteoadherin, Oxytocin-neurophysin 1, p16 ARC , P20Sb3, p23, p39 , P4HB, p73, PA2G4, PABP, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCDH7, PCDX8, PCK2 , PCMT1, PCNA, PCPE-1, Proprotein Convertase 9, PCYOX1, PDE1B, PDIA6, PDLIM1, PDLIM5, PDZD2, PEBP4, PEPD, PER1, Perilipin-3, Perilipin-1 , Periostin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin 5, PF4V1, PGAM1, PGAM2, PGD, PGDF, PGK-1, PGLS-C-t, PGM1, PGRPL, PHAP1, Phosphoserine Aminotransferase, PI3-Kinase C2 beta, pIgR, PIK3IP1, PIN, PISD, PKLR, PLA2G1B, Plakophilin 1, Plastin L, PLC-gamma 1 , Pleckstrin, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, PLS3, Plxdc2, PNP, POR, PPCS, PPOX , PPP2R1B , PPP2R4, PRCP, PRDM13, PRDX 1, PRELP, PREP, PRG2, Prion protein PrP , Profilin 1, Properdin, Prosaposin, Prostaglandin D Synthase, Proteasome 20S a+b, Proteasome 20S alpha, Proteasome 20S alpha 5, Proteasome 20S b7, Proteasome 26S S5, Proteasome beta 1, Proteasome subunit alpha type 6, Proteasome subunit beta type 2, Proteasome subunit beta type 4, Coagulation Factor XIV, Protein Z, Prouroguanylin, PRSS23, PRSS3, Myeloblastin, PSMA1, PSMA2, PSMA4, PSMA7, PSMB5, PSMC3 , PSMD1, PSMD9 , PTEN, PTK 7, PTMA, PTP gamma, PTP kappa, PTP mu, PTPRS, PTPRZ, PYGL, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, RAP1AB, Rbm15, RCL , Reg1A, Reg3A, RHOC, RhoGDI, Ribonuclease A, Ribonuclease Inhibitor, Ribonuclease T2, RKIP, RNA Polymerase II, RNASE4, RNASE6, RPL10, RPL10A , RPL11, RPL12, RPL14 , RPL17, RPL22, RPL5, RPL7A, RPLP0 , RPS10, RPS11, RPS12, RPS19, RPS2, RPS20 , RPS23, RPS25, RPS28, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A11, S100 A7, S100 A9, S100P, SAA, SAA4a, Salivary alpha amylase, SAMSN1, SBP-1, SBSN, SDF4, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Semenogelin I, Semenogelin II, Serpin A11, Serpin A7, Serpin B3, Serpin B6, Serpin B8, Serpin F2, Serpin A10, Serpin B1, Serpin B4, Serpin E2, SerRS, SET, SEZ6L2, SF20, SH3BGRL, SH3BGRL3, SHANK1, SHC1, SHIP, SHMT1, SHP-1, Siglec-1, Siglec-14, SIM2 , SIRP beta 1, Six3, SLC38A10, SLITRK1, SLURP1, SMA, SMC4 , SMPD4, SOD1, SOD2, SOD3, SOD4, Somatostatin, SORD, SorLA, SOX4, SP-D, Spectrin beta-5, SPEN, SPINK7, SPTBN1, Src(1), SREC-II, STAT3, Stathmin 1, Sterol carrier protein 2, STI1, STOM, SUCLG1, SUMO3, Symplekin, SynCAM, Syntaxin 7, TAB182, TAGLN2, Talin1, Talin1&2, TAX1BP3 , TBCA, TCEB2, Tcf20, TCN1, TCP1 eta, Tenascin C, Tenascin X(1), TFF2(1), TGM3, Thioredoxin-1, Thimet Oligopeptidase, Thymosin b10, Titin, TLS, TMEM223, TOB2 , TOP2B, TPM4, Tripeptidyl-peptidase I, Transaldolase 1, Transketolase, Transthyretin, TRAP1, TRAP220, TRF 2, Triosephosphate isomerase, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TrypsinPan, Tryptophanyl, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UBE2N, Ubiquitin+1, UCH-L1, UFM 1, UGGT, UNC-13 Homolog D, UNC45A, UNC5H4, UPB1 , UQCRB, UQCRH , URB, URB2, UROC1, UROD, URP2, USP14 , USP5 , Uteroglobin, Utrophin, VAP-1, VAP-A, VCP, VDAC1 , Versican isoform V0, Vimentin B, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1 , YY1, ZBTB4, ZC3H4-N-t, ZC3H8, ZDHHC18 , ZNF671, Zyxin
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Protein Tyrosine Phosphorylation Antibody Array G4 Slide(s)
- 2X Cell Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set II
- Blocking Buffer
- 20X Wash Buffer I
- 20X Wash Buffer II
- Biotin-Conjugated Anti-phosphotyrosine
- Fluorescent dye-Conjugated Streptavidin
- Wash Buffer III
- Adhesive film
Other Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 ml Centrifuge tube
- Isopropanol (2-propanol)
Protocol Outline
- Dry the Glass Slide
- Blocking and Incubation
- Fluorescence Detection
Storage/Stability
Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.