Format Selection / Instrument Key

Format selection is based on the plate type compatible with your instrument model. Below are the compatible instruments for each plate type.

Introduction

Recent discoveries in RNA modification–mediated gene regulation have fueled the rapidly expanding field of epitranscriptomics. Messenger RNAs (mRNAs), along with transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), undergo dynamic internal modifications such as N1-methyladenosine (m¹A), N6-methyladenosine (m⁶A), N7-methylguanosine (m⁷G), 5-methylcytosine (m⁵C), N4-acetylcytidine (ac⁴C), adenosine-to-inosine (A-to-I), and pseudouridine (Ψ). These chemical marks serve as critical regulators of RNA stability, processing, translation, and overall metabolism. The deposition, removal, and recognition of RNA methylation are carried out by three major classes of proteins: "writers" (methyltransferases), "erasers" (demethylases), and "readers" (RNA-binding proteins). Increasing evidence links dysregulation of these regulators to a broad spectrum of human diseases and disorders.

The RayBio^® Human RNA Modification qPCR Array is a SYBR Green-based qPCR assay that enables simultaneous profiling of 90 validated regulators across nine classes of RNA epitranscriptomic modifications. This kit contains six controls: three reference genes (internal control, INC) for data normalization, a genomic DNA (gDNA) control (GDC) for detection of possible gDNA contamination, a positive control (PC) for PCR amplification confirmation, and a negative control (NC) to monitor DNA contamination during workflow. Each 96-well plate is preloaded with a rigorously validated, highly specific primer pair to ensure consistent and reliable results. Simply add cDNA and qPCR master mix, run the assay, and analyze the expression profiles to uncover differentially expressed genes. Delivering high-quality results in as little as two hours, this PCR Array provides a rapid, convenient, and powerful tool for exploring the dynamic regulatory landscape of the epitranscriptome.

Product Features

Kit Components

Additional Materials Required

Contact our technical support team for questions about compatibility: techsupport@raybiotech.com

Assay Procedure Summary

Storage/Stability

The kit can be stored at -20°C for a period of 24 months prior to opening. Avoid repeated freeze-thaw cycles.