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Hydroxysteroid Dehydrogenase

Catalog #: 255-10048

A purified powder obtained from adapted cells of a mutant strain. Activity on androsterone only, no activity exhibited on testosterone.

Product Description

Specifications

Size10 un, 50 un
Estimated Lead Time1-2 weeks
Expression SystemPseudomonas testosteroni
Molecular Weight (kDa)
100,000 (Squire et al. 1964)
Activity
≥0.5 units per mg dry weight
Research AreaEndocrine
SourcePseudomonas testosteroni
Shipping TypeBlue ice
Storage-20°C

Description

Other Information

Isoelectric
IsoelecttricpH 6.5 (Squire et al. 1964). Specificity: Catalyzes the oxidation of 3β-hydroxysteroids of the C19 and C21 series, 17β-hydroxysteroids of the C18, C19, and C21 series, as well as certain 16β-hydroxysteroids (Talalay 1963). Inhibitors: Heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17α-estradiol and other 1,3,5-estratriene derivatives (Talalay 1963). Stability: The preparation of mixed α and β enzyme is stable for 6-12 months when stored at -20°C.
CAS Registry Number
C.A.S.: 9028-56-2 and 9015-81-0
Storage Conditions
Store at -20°C.