RayBiotech provides fixed cells containing the overexpressed protein-of-interest, which can be used as positive controls in immunohistochemistry (IHC) experiments or to validate antibodies for IHC use. The cells are provided as a pellet, which can then be cut into sections for IHC analysis (see Figure 1, "General IHC Steps").
IHC is a valuable tool in research, clinical diagnosis, and drug development since it enables the visualization of one or more proteins-of-interest in cells or tissues. However, obtaining IHC positive controls can be difficult, particularly when the protein-of-interest is produced by a pathogen.
If your gene-of-interest (GOI) is not available, we will synthesize the GOI with codon optimization that is appropriate for the expression system. Larger genes will result in longer lead times.
Lead time: 2 – 4 weeks
Deliverables: Gene will remain at RayBiotech for Phase 2. If desired, the gene can also be shipped to the customer.
GOI is cloned into an expression vector specific for the expression system. Proper insertion of the gene is confirmed via DNA sequencing.
Lead time: 1 – 2 weeks
Deliverables: Cloned gene will remain at RayBiotech for Phase 3.
Expression of GOI is verified with a western blot using a small number of cells.
Lead time: 1 week
Deliverables: Customer will receive the western blot image. Gene sequencing data available upon request.
GOI is expressed in ∼90 million cells. Cells are pelleted and fixed.
Lead time: 2 weeks
Deliverables: Pellet of fixed cells will be shipped to customer with ice.