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An indirect Enzyme-Linked Immunosorbent Assay (iELISA) is a common biochemical technique that detects an antibody using a labeled secondary antibody. This assay is primarily used to detect the total antibody amount in a sample. This method is commonly utilized to diagnose infection and to quantify antibodies against an invading antigen.
Don’t see a pre-made kit that fits your needs? We can customize one for you. Detect the presence of targeted antibodies in your sample with customized kit, optimized by experienced biotechnology professionals. We can send the newly developed kit to you or run it in house with a certified service. Check out the example manual for more information.
RayBio iELISAs employ a specific antigen-coated 96-well plate. A sample containing the primary antibody is incubated into the wells and the antibodies present bind to the immobilized antigen. Next, the wells are washed and a biotin-labeled detection antibody that recognizes the primary antibody is added. After washing away unbound antibodies, an HRP-conjugated secondary antibody is then added, and when combined with a substrate, produces a signal amplification that is directly proportional to the amount of bound sample antibody.