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Interleukin-3, Mouse Recombinant
Catalog #: 228-10919
Product Description
Specifications
| Size | 10 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Mouse |
| Accession Number | P01586 |
| Gene Id | 16187 |
| Protein Name / Synonyms | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg. |
| Expression System | E. coli |
| Purity | Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. |
| Activity | The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is <, 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg. |
| Research Area | Inflammation, Autophagy |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.
Specifications
Protein Content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.