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Leptin, Rat Recombinant
Catalog #: 228-11021
Product Description
Specifications
| Size | 5 mg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Rat |
| Accession Number | P50596 |
| Gene Id | 25608 |
| Protein Name / Synonyms | OB Protein, Obesity Protein, OBS, Obesity factor. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-His. |
| Expression System | E. coli |
| Purity | Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Activity | Biological activity of Leptin Rat is evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| Reconstitution | It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Leptin Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques.
Specifications
Protein Content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.201 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of Leptin rat as a Reference Standard.
Stability & Storage
Storage/Stability
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.