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ISO 13485:2016 cGMP
Listeriolysin-O Recombinant
Catalog #: 228-11051
Product Description
Specifications
| Size | 50 µg, 100 µg |
| Estimated Lead Time | 2-3 weeks |
| Accession Number | Q9L5B9 |
| Protein Name / Synonyms | Listeriolysin-O, LLO, hlyA. |
| Expression System | E. coli |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Format | Sterile Filtered clear solution. |
| Formulation | The protein contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% (v/v) ,glycerol and 0.5M NaCl. |
| Activity | Hemolytic activity for Recombinant Listeriolysin O was 1.18 x 105 HU/mg of protein where HU means hemolytic activity unit that is the amount of toxin needed to release half the hemoglobin (50% lysis) of the erythrocytes as determined by hemolysin assay.2m |
| Recommended Applications | a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6). |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
Molecular weight of Listeriolysin O is 58kDa. This haemolysin protein is provided in its mature form that is constituted by 503 amino acids, from 26 to 529 of the registered sequence (accession number: AAF64524).
Stability & Storage
Storage/Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.