Monkeypox Virus A29L Protein Human IgG ELISA Kit

The Monkeypox Virus (MPXV) A29L Protein Human IgG ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgG antibody against MPXV A29L in human serum or plasma. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Monkeypox Virus
Compatible Sample Types	Plasma, Serum
Gene Symbols	IGG
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Indirect
Method Of Detection	Colorimetric
Research Area	Infectious Disease, Mpox
Shipping Type	Dry ice (additional shipping fee)
Storage	-80°C

Application Notes

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Detection Antibody
Biotinylated Anti-Positive Control
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Application Notes

Kit Components

Pre-coated 96-well microplate
Wash Buffer
Positive Control
Biotinylated Detection Antibody
Biotinylated Anti-Positive Control
HRP-Streptavidin concentrate
TMB One-Step Substrate
Stop Solution
Assay Diluent
5X Sample Diluent

Other Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water

Protocol Outline

Prepare all reagents, samples and standards as instructed.
Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µl Stop Solution to each well. Read at 450 nm immediately.

Featured Products

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Have this product in hand?

Featured Products

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Have this product in hand?

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Featured Products

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Product Description

Specifications

Application Notes

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Monkeypox Virus A29L Protein Human IgG ELISA Kit

Monkeypox Virus A29L Protein Human IgG ELISA Kit