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Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1000 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Mouse |
| Accession Number | P07437 |
| Gene Id | 203068 |
| Gene Symbols | TUBB TUBB5 OK/SW-cl.56 |
| Protein Name / Synonyms | Beta-tubulin (166-183aa) |
| Clonality | Monoclonal |
| Clone | 3B7-B12-B9 |
| Immunogen | Immunogen was polypeptide Beta-tubulin (166-183aa) - KLH:CTFSVVPSPKVSDTVVEPY. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Immunogen Sequence | KLH:CTFSVVPSPKVSDTVVEPY |
| Recommended Applications | ELISA (recommended work dilution = 1:320, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Western Blot
130-10983 (mAb): 1:2000
10 % SDS-PAGE
Goat anti-mouse IgG-HRP: 1:10,000
Lane M: Protein marker 5 µl
2: K562 cytoplasm 5 µl
3: MCF7 cytoplasm 5 µl
Simple Western
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.