Mouse Anti-GFP Tag (Ascites)

Mouse Anti-GFP Tag (Ascites) Antibody, 0.1 ml
In stock

Product Description


Size 0.1 ml
Protein Name / Synonyms GFP Tag (Ascites)
Preparation Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Clonality Monoclonal


Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. RayBiotech's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. RayBiotech's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.


Other Names
Green Fluorescent Protein


Mouse Monoclonal Antibody (Mab)
Recommended Dilutions
WB: 1:100-500
Western blot analysis of anti-GFP Mab (Cat# 102-10010) using purified GFP, YFP and BFP proteins expressed in bacteria: Both GFP (Lanes 1-3) and YFP (Lanes 4-6) but not BFP (data not shown) were detected using the purified Mab.
Western blot analysis of anti-GFP Tag Antibody (Ascites) (CA071114E) in GFP recombinant protein. GFP recombinant protein (arrow) was detected using the purified Mab.


2-8°C (short-term); -20°C (long-term)
12 months from the date of shipment when stored properly.
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