Mouse Anti-Human CD33 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P20138 |
| Gene Id | 945 |
| Gene Symbols | CD33 |
| Protein Name / Synonyms | CD33 |
| Clonality | Monoclonal |
| Preparation | This CD33 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from human CD33. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Specificity
Other Names
CD33; SIGLEC3; Myeloid cell surface antigen CD33; Sialic acid-binding Ig-like lectin 3; gp67; CD_antigen=CD33; Flags: Precursor
NCBI Accession #
NP_001076087.1;NP_001171079.1;NP_001763.3
Other Accession #
NP_001763.3
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
39825
Recommended Dilutions
IF: 1:10-50; WB: 1:100-500
 | Fluorescent image of A549 cell stained with CD33 Antibody(Cat# 102-10371/SM120504AC).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CD33 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).CD33 immunoreactivity is localized to Membrane significantly. |
 | CD33 Antibody(Cat# 102-10371) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.